Cyclin-Dependent Kinase Inhibitor Gene TaICK1 acts as a Potential Contributor to Wheat Male Sterility induced by a Chemical Hybridizing Agent
Abstract
:1. Introduction
2. Results
2.1. Morphological and Cytological Characteristics
2.2. Structure of the Gene TaICK1
2.3. Expression Level of TaICK1 in XN1376-CIMS and XN1376
2.4. The Phenotype of TaICK1 Transgenic Rice Plants
2.5. Expression Patterns of TaICK1 and OsICK1 in Transgenic Rice
2.6. Subcellular Localization Assay of Protein TaICK1
2.7. Prokaryotic Expression of TaICK1 in E. coli
2.8. TaICK1 Interacts with TaCYCD2;1 and TaCYCD6;1
2.9. The Expression Patterns of CYCD2;1 and CYCD6;1 in Wheat and Transgenic Rice
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Phenotype Characterization of Wheat Anther Development
4.3. RNA Extraction and qRT-PCR Analysis
4.4. Primer Design
4.5. Plasmid Constructs
4.6. Generation of the TaICK1 Overexpression Rice Plants
4.7. Yeast Two-Hybrid Assay
4.8. Subcellular Localization and BiFC Assay
4.9. Recombinant Protein Expression in E. coli
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Plant | OE-1 | OE-3 | OE-5 | OE-7 | OE-8 | OE-10 | OE-15 | WT |
---|---|---|---|---|---|---|---|---|
T1 | 19.7% | 58.6% | 58.7% | 63.5% | 38.1% | 57.4% | 29.6% | 94.1% |
T2 | 33.4% | 58.6% | 66.2% | 46.7% | 47.3% | 30.2% | 68.9% | 95.1% |
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Zhang, L.; Wang, C.; Yu, Y.; Zhang, Y.; Song, Y.; Li, Z.; Wang, S.; Zhang, Y.; Guo, X.; Liu, D.; et al. Cyclin-Dependent Kinase Inhibitor Gene TaICK1 acts as a Potential Contributor to Wheat Male Sterility induced by a Chemical Hybridizing Agent. Int. J. Mol. Sci. 2020, 21, 2468. https://doi.org/10.3390/ijms21072468
Zhang L, Wang C, Yu Y, Zhang Y, Song Y, Li Z, Wang S, Zhang Y, Guo X, Liu D, et al. Cyclin-Dependent Kinase Inhibitor Gene TaICK1 acts as a Potential Contributor to Wheat Male Sterility induced by a Chemical Hybridizing Agent. International Journal of Molecular Sciences. 2020; 21(7):2468. https://doi.org/10.3390/ijms21072468
Chicago/Turabian StyleZhang, Lili, Chaojie Wang, Yongang Yu, Yamin Zhang, Yulong Song, Zheng Li, Shuping Wang, Yanfang Zhang, Xiaofeng Guo, Dan Liu, and et al. 2020. "Cyclin-Dependent Kinase Inhibitor Gene TaICK1 acts as a Potential Contributor to Wheat Male Sterility induced by a Chemical Hybridizing Agent" International Journal of Molecular Sciences 21, no. 7: 2468. https://doi.org/10.3390/ijms21072468