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Article

Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter

1
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan, China
2
Post-Doctoral Research Station of Plant Protection as first class discipline, Yunnan Agricultural University, Kunming 650201, Yunnan, China
3
Rice Research Institute, Yunnan Agricultural University, Kunming 650201, Yunnan, China
4
Yunnan Engineering Research Center for Japonica Hybrid Rice, Kunming 650201, Yunnan, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(6), 1909; https://doi.org/10.3390/ijms21061909
Received: 17 January 2020 / Revised: 6 March 2020 / Accepted: 9 March 2020 / Published: 11 March 2020
(This article belongs to the Section Molecular Plant Sciences)
The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of different putative regulatory motifs in the OsSUT3 promoter, a series of promoter deletions were fused to a GUS reporter gene and then stably introduced into rice and Arabidopsis. Histochemical GUS analysis of transgenic plants revealed that p385 (from −385 to −1) specifically mediated maximal GUS expression in pollen tissues. The S region (from −385 to −203) was the key region for controlling the pollen-specific expression of a downstream gene. The E1 (−967 to −606), E2 (−202 to −120), and E3 (−119 to −1) regions enhanced ectopic promoter activity to different degrees. Moreover, the p385 promoter could alter the expression pattern of the 35S promoter and improve its activity when they were fused together. In summary, the p385 promoter, a short and high-activity promoter, can function to drive pollen-specific expression of transgenes in monocotyledon and dicotyledon transformation experiments. View Full-Text
Keywords: rice; pollen-specificity; expression; OsSUT3; promoter rice; pollen-specificity; expression; OsSUT3; promoter
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MDPI and ACS Style

Li, D.; Xu, R.; Lv, D.; Zhang, C.; Yang, H.; Zhang, J.; Wen, J.; Li, C.; Tan, X. Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter. Int. J. Mol. Sci. 2020, 21, 1909. https://doi.org/10.3390/ijms21061909

AMA Style

Li D, Xu R, Lv D, Zhang C, Yang H, Zhang J, Wen J, Li C, Tan X. Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter. International Journal of Molecular Sciences. 2020; 21(6):1909. https://doi.org/10.3390/ijms21061909

Chicago/Turabian Style

Li, Dandan, Rucong Xu, Dong Lv, Chunlong Zhang, Hong Yang, Jianbo Zhang, Jiancheng Wen, Chengyun Li, and Xuelin Tan. 2020. "Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter" International Journal of Molecular Sciences 21, no. 6: 1909. https://doi.org/10.3390/ijms21061909

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