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Article

Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis

Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
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Int. J. Mol. Sci. 2020, 21(5), 1801; https://doi.org/10.3390/ijms21051801
Received: 16 January 2020 / Revised: 1 March 2020 / Accepted: 2 March 2020 / Published: 5 March 2020
(This article belongs to the Collection Stress, Immunity, and Tissue Microenvironment)
Interleukin (IL)-33 is a proinflammatory mediator that alerts the host immune system to disorders in tissue homeostasis. Aim. To understand the role of IL-33 in modulating gastric tissue cell growth affected by Helicobacter pylori (H. pylori). Methods. IL-33 production in guinea pigs (Caviae porcellus) experimentally infected with H. pylori was evaluated by ELISA or immunohistochemical staining. The proregenerative activity of IL-33 was evaluated using gastric epithelial cells and fibroblasts that were naive or transfected with IL-33 siRNA exposed to H. pylori glycine acid extract antigenic complex (GE), as well as by measuring cell migration, proliferation, metabolic activity and apoptosis. Animals infected by H. pylori responded with increased production of IL-33. Also, cells treated in vitro with GE released more IL-33 than cells that were unstimulated. Silencing IL-33 in cells resulted in downregulation of metabolic activity, adhesion, migration and proliferation, especially after treatment with H. pylori GE, as well as upregulation of cells apoptosis associated with caspase 3 increase and Bcl-xL decrease, suggesting proregenerative activity of IL-33. Interestingly, upregulation of cell proliferation by IL-33 was Erk independent. Our results indicate that IL-33 may protect gastric tissue from loss of homeostasis caused by deleterious effects of H. pylori components and the inflammatory response developed during infection. View Full-Text
Keywords: Helicobacter pylori; gastric barrier; IL-33; proliferation; apoptosis Helicobacter pylori; gastric barrier; IL-33; proliferation; apoptosis
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MDPI and ACS Style

Gonciarz, W.; Krupa, A.; Chmiela, M. Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis. Int. J. Mol. Sci. 2020, 21, 1801. https://doi.org/10.3390/ijms21051801

AMA Style

Gonciarz W, Krupa A, Chmiela M. Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis. International Journal of Molecular Sciences. 2020; 21(5):1801. https://doi.org/10.3390/ijms21051801

Chicago/Turabian Style

Gonciarz, Weronika, Agnieszka Krupa, and Magdalena Chmiela. 2020. "Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis" International Journal of Molecular Sciences 21, no. 5: 1801. https://doi.org/10.3390/ijms21051801

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