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The Cannabis Proteome Draft Map Project

Department of Biology, Hood College, Frederick, MD 21701, USA
Think20 Labs, Columbia, MD 21046, USA
Department of Core Facilities, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 965;
Received: 12 November 2019 / Revised: 21 January 2020 / Accepted: 24 January 2020 / Published: 31 January 2020
(This article belongs to the Collection Advances in Proteomic Research)
Recently we have seen a relaxation of the historic restrictions on the use and subsequent research on the Cannabis plants, generally classified as Cannabis sativa and Cannabis indica. What research has been performed to date has centered on chemical analysis of plant flower products, namely cannabinoids and various terpenes that directly contribute to phenotypic characteristics of the female flowers. In addition, we have seen many groups recently completing genetic profiles of various plants of commercial value. To date, no comprehensive attempt has been made to profile the proteomes of these plants. We report herein our progress on constructing a comprehensive draft map of the Cannabis proteome. To date we have identified over 17,000 potential protein sequences. Unfortunately, no annotated genome of Cannabis plants currently exists. We present a method by which “next generation” DNA sequencing output and shotgun proteomics data can be combined to produce annotated FASTA files, bypassing the need for annotated genetic information altogether in traditional proteomics workflows. The resulting material represents the first comprehensive annotated protein FASTA for any Cannabis plant. Using this annotated database as reference we can refine our protein identifications, resulting in the confident identification of 13,000 proteins with putative function. Furthermore, we demonstrate that post-translational modifications play an important role in the proteomes of Cannabis flower, particularly lysine acetylation and protein glycosylation. To facilitate the evolution of analytical investigations into these plant materials, we have created a portal to host resources developed from our proteomic and metabolomic analysis of Cannabis plant material as well as our results integrating these resources. View Full-Text
Keywords: Proteomics; proteogenomics; Cannabis; PTMs Proteomics; proteogenomics; Cannabis; PTMs
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MDPI and ACS Style

Jenkins, C.; Orsburn, B. The Cannabis Proteome Draft Map Project. Int. J. Mol. Sci. 2020, 21, 965.

AMA Style

Jenkins C, Orsburn B. The Cannabis Proteome Draft Map Project. International Journal of Molecular Sciences. 2020; 21(3):965.

Chicago/Turabian Style

Jenkins, Conor, and Benjamin Orsburn. 2020. "The Cannabis Proteome Draft Map Project" International Journal of Molecular Sciences 21, no. 3: 965.

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