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In Vivo Imaging with Genetically Encoded Redox Biosensors
Open AccessArticle

Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity

1
Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia
2
Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia
3
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
4
Synthetic Biology Group, MRC London Institute of Medical Sciences, London W12 0NN, UK
5
Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London W12 0NN, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8800; https://doi.org/10.3390/ijms21228800
Received: 11 October 2020 / Revised: 9 November 2020 / Accepted: 17 November 2020 / Published: 20 November 2020
Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures. View Full-Text
Keywords: optogenetics; genetically encoded photosensitizers; fluorescent proteins; phototoxicity; KillerRed; SuperNova optogenetics; genetically encoded photosensitizers; fluorescent proteins; phototoxicity; KillerRed; SuperNova
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MDPI and ACS Style

Gorbachev, D.A.; Staroverov, D.B.; Lukyanov, K.A.; Sarkisyan, K.S. Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity. Int. J. Mol. Sci. 2020, 21, 8800. https://doi.org/10.3390/ijms21228800

AMA Style

Gorbachev DA, Staroverov DB, Lukyanov KA, Sarkisyan KS. Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity. International Journal of Molecular Sciences. 2020; 21(22):8800. https://doi.org/10.3390/ijms21228800

Chicago/Turabian Style

Gorbachev, Dmitry A.; Staroverov, Dmitry B.; Lukyanov, Konstantin A.; Sarkisyan, Karen S. 2020. "Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity" Int. J. Mol. Sci. 21, no. 22: 8800. https://doi.org/10.3390/ijms21228800

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