Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity
1
Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia
2
Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia
3
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
4
Synthetic Biology Group, MRC London Institute of Medical Sciences, London W12 0NN, UK
5
Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London W12 0NN, UK
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8800; https://doi.org/10.3390/ijms21228800
Received: 11 October 2020 / Revised: 9 November 2020 / Accepted: 17 November 2020 / Published: 20 November 2020
(This article belongs to the Special Issue Advanced Fluorescent Probes for Structural and Functional Imaging of Biological Systems)
Genetically encoded photosensitizers are increasingly used as optogenetic tools to control cell fate or trigger intracellular processes. A monomeric red fluorescent protein called SuperNova has been recently developed, however, it demonstrates suboptimal characteristics in most phototoxicity-based applications. Here, we applied directed evolution to this protein and identified SuperNova2, a protein with S10R substitution that results in enhanced brightness, chromophore maturation and phototoxicity in bacterial and mammalian cell cultures.
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Keywords:
optogenetics; genetically encoded photosensitizers; fluorescent proteins; phototoxicity; KillerRed; SuperNova
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MDPI and ACS Style
Gorbachev, D.A.; Staroverov, D.B.; Lukyanov, K.A.; Sarkisyan, K.S. Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity. Int. J. Mol. Sci. 2020, 21, 8800. https://doi.org/10.3390/ijms21228800
AMA Style
Gorbachev DA, Staroverov DB, Lukyanov KA, Sarkisyan KS. Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity. International Journal of Molecular Sciences. 2020; 21(22):8800. https://doi.org/10.3390/ijms21228800
Chicago/Turabian StyleGorbachev, Dmitry A.; Staroverov, Dmitry B.; Lukyanov, Konstantin A.; Sarkisyan, Karen S. 2020. "Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity" Int. J. Mol. Sci. 21, no. 22: 8800. https://doi.org/10.3390/ijms21228800
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