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Open AccessArticle

Detergent Resistant Membrane Domains in Broccoli Plasma Membrane Associated to the Response to Salinity Stress

1
Group of Aquaporins, Department of Plant Nutrition, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus de Espinardo, E-30100 Murcia, Spain
2
Department of Agronomy Engineering Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203 Cartagena (Murcia), Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(20), 7694; https://doi.org/10.3390/ijms21207694
Received: 25 September 2020 / Accepted: 13 October 2020 / Published: 17 October 2020
(This article belongs to the Section Molecular Plant Sciences)
Detergent-resistant membranes (DRMs) microdomains, or “raft lipids”, are key components of the plasma membrane (PM), being involved in membrane trafficking, signal transduction, cell wall metabolism or endocytosis. Proteins imbibed in these domains play important roles in these cellular functions, but there are few studies concerning DRMs under abiotic stress. In this work, we determine DRMs from the PM of broccoli roots, the lipid and protein content, the vesicles structure, their water osmotic permeability and a proteomic characterization focused mainly in aquaporin isoforms under salinity (80 mM NaCl). Based on biochemical lipid composition, higher fatty acid saturation and enriched sterol content under stress resulted in membranes, which decreased osmotic water permeability with regard to other PM vesicles, but this permeability was maintained under control and saline conditions; this maintenance may be related to a lower amount of total PIP1 and PIP2. Selective aquaporin isoforms related to the stress response such as PIP1;2 and PIP2;7 were found in DRMs and this protein partitioning may act as a mechanism to regulate aquaporins involved in the response to salt stress. Other proteins related to protein synthesis, metabolism and energy were identified in DRMs independently of the treatment, indicating their preference to organize in DMRs. View Full-Text
Keywords: aquaporins; broccoli; detergent-resistant membrane; microdomains; plasma membrane; salt stress aquaporins; broccoli; detergent-resistant membrane; microdomains; plasma membrane; salt stress
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MDPI and ACS Style

Yepes-Molina, L.; Carvajal, M.; Martínez-Ballesta, M.C. Detergent Resistant Membrane Domains in Broccoli Plasma Membrane Associated to the Response to Salinity Stress. Int. J. Mol. Sci. 2020, 21, 7694.

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