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Open AccessArticle

Alterations in Anandamide Synthesis and Degradation during Osteoarthritis Progression in an Animal Model

Maj Institute of Pharmacology, Polish Academy of Sciences, 31-343 Cracow, Poland
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Author to whom correspondence should be addressed.
Current address: Endocannabinoid Research Group, Institute of Biomolecular Chemistry, National Research Council of Italy, Via Campi Flegrei 34, 80078 Napoli, Italy; Institute of Genetics and Biophysics, National Research Council of Italy, Via Pietro Castellino 111, 80131 Napoli, Italy.
Int. J. Mol. Sci. 2020, 21(19), 7381; https://doi.org/10.3390/ijms21197381
Received: 4 September 2020 / Revised: 28 September 2020 / Accepted: 3 October 2020 / Published: 6 October 2020
Osteoarthritis (OA) is a degenerative joint disease manifested by movement limitations and chronic pain. Endocannabinoid system (ECS) may modulate nociception via cannabinoid and TRPV1 receptors. The purpose of our study was to examine alterations in the spinal and joint endocannabinoid system during pain development in an animal model of OA. Wistar rats received intra-articular injection of 3mg of sodium monoiodoacetate (MIA) into the knee joint. Animals were sacrificed on day 2, 7, 14, 21, 28 after injection and lumbar spinal cord, cartilage and synovium were collected. Changes in the transcription levels of the ECS elements were measured. At the spinal level, gene expression levels of the cannabinoid and TRPV1 receptors as well as enzymes involved in anandamide synthesis and degradation were elevated in the advanced OA phase. In the joint, an important role of the synovium was demonstrated, since cartilage degeneration resulted in attenuation of the changes in the gene expression. Enzymes responsible for anandamide synthesis and degradation were upregulated particularly in the early stages of OA, presumably in response to early local joint inflammation. The presented study provides missing information about the MIA-induced OA model and encourages the development of a therapy focused on the molecular role of ECS. View Full-Text
Keywords: osteoarthritis; endocannabinoid system; endocannabinoids; pain; chronic pain; TRPV1; animal model; cartilage; synovial membrane; spinal cord osteoarthritis; endocannabinoid system; endocannabinoids; pain; chronic pain; TRPV1; animal model; cartilage; synovial membrane; spinal cord
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Bryk, M.; Chwastek, J.; Kostrzewa, M.; Mlost, J.; Pędracka, A.; Starowicz, K. Alterations in Anandamide Synthesis and Degradation during Osteoarthritis Progression in an Animal Model. Int. J. Mol. Sci. 2020, 21, 7381.

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