Next Article in Journal
Effects of Arbuscular Mycorrhization on Fruit Quality in Industrialized Tomato Production
Next Article in Special Issue
Docosahexaenoic Acid-Loaded Polylactic Acid Core-Shell Nanofiber Membranes for Regenerative Medicine after Spinal Cord Injury: In Vitro and In Vivo Study
Previous Article in Journal
Macular Ganglion Cell Complex and Peripapillary Retinal Nerve Fiber Layer Thinning in Patients with Type-1 Gaucher Disease
Previous Article in Special Issue
Influence of the Casting Concentration on the Mechanical and Optical Properties of FA/CaCl2-Derived Silk Fibroin Membranes
Article

BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model

1
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
2
Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
3
Department of Biomaterials, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
4
Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama 700-8558, Japan
5
Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7028; https://doi.org/10.3390/ijms21197028
Received: 26 August 2020 / Revised: 14 September 2020 / Accepted: 22 September 2020 / Published: 24 September 2020
Medication-related osteonecrosis of the jaw (MRONJ) is a severe pathological condition associated mainly with the long-term administration of bone resorption inhibitors, which are known to induce suppression of osteoclast activity and bone remodeling. Bone Morphogenetic Protein (BMP)-2 is known to be a strong inducer of bone remodeling, by directly regulating osteoblast differentiation and osteoclast activity. This study aimed to evaluate the effects of BMP-2 adsorbed onto beta-tricalcium phosphate (β-TCP), which is an osteoinductive bioceramic material and allows space retention, on the prevention and treatment of MRONJ in mice. Tooth extraction was performed after 3 weeks of zoledronate (ZA) and cyclophosphamide (CY) administration. For prevention studies, BMP-2/β-TCP was transplanted immediately after tooth extraction, and the mice were administered ZA and CY for an additional 4 weeks. The results showed that while the tooth extraction socket was mainly filled with a sparse tissue in the control group, bone formation was observed at the apex of the tooth extraction socket and was filled with a dense connective tissue rich in cellular components in the BMP-2/β-TCP transplanted group. For treatment studies, BMP-2/β-TCP was transplanted 2 weeks after tooth extraction, and bone formation was followed up for the subsequent 4 weeks under ZA and CY suspension. The results showed that although the tooth extraction socket was mainly filled with soft tissue in the control group, transplantation of BMP-2/β-TCP could significantly accelerate bone formation, as shown by immunohistochemical analysis for osteopontin, and reduce the bone necrosis in tooth extraction sockets. These data suggest that the combination of BMP-2/β-TCP could become a suitable therapy for the management of MRONJ. View Full-Text
Keywords: BMP-2; MRONJ; bone regeneration BMP-2; MRONJ; bone regeneration
Show Figures

Figure 1

MDPI and ACS Style

Mikai, A.; Ono, M.; Tosa, I.; Nguyen, H.T.T.; Hara, E.S.; Nosho, S.; Kimura-Ono, A.; Nawachi, K.; Takarada, T.; Kuboki, T.; Oohashi, T. BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model. Int. J. Mol. Sci. 2020, 21, 7028. https://doi.org/10.3390/ijms21197028

AMA Style

Mikai A, Ono M, Tosa I, Nguyen HTT, Hara ES, Nosho S, Kimura-Ono A, Nawachi K, Takarada T, Kuboki T, Oohashi T. BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model. International Journal of Molecular Sciences. 2020; 21(19):7028. https://doi.org/10.3390/ijms21197028

Chicago/Turabian Style

Mikai, Akihiro, Mitsuaki Ono, Ikue Tosa, Ha T.T. Nguyen, Emilio S. Hara, Shuji Nosho, Aya Kimura-Ono, Kumiko Nawachi, Takeshi Takarada, Takuo Kuboki, and Toshitaka Oohashi. 2020. "BMP-2/β-TCP Local Delivery for Bone Regeneration in MRONJ-Like Mouse Model" International Journal of Molecular Sciences 21, no. 19: 7028. https://doi.org/10.3390/ijms21197028

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop