Next Article in Journal
verA Gene is Involved in the Step to Make the Xanthone Structure of Demethylsterigmatocystin in Aflatoxin Biosynthesis
Next Article in Special Issue
Preconditioned and Genetically Modified Stem Cells for Myocardial Infarction Treatment
Previous Article in Journal
Siloxanes—Versatile Materials for Surface Functionalisation and Graft Copolymers
Previous Article in Special Issue
Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Improves Energetic Status and Cardiomyogenic Differentiation of Human Dilated Myocardium-Derived Primary Mesenchymal Cells
Open AccessReview

Human Cell Modeling for Cardiovascular Diseases

1
Unit of Vascular Biology and Regenerative Medicine, Centro Cardiologico Monzino IRCCS, 20138 Milan, Italy
2
Department of Clinical Sciences and Community Health, Università degli Studi di Milano, 20122 Milan, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6388; https://doi.org/10.3390/ijms21176388
Received: 13 August 2020 / Revised: 28 August 2020 / Accepted: 31 August 2020 / Published: 2 September 2020
(This article belongs to the Special Issue Cell Programming for Cardiovascular Disease Modeling and Therapy)
The availability of appropriate and reliable in vitro cell models recapitulating human cardiovascular diseases has been the aim of numerous researchers, in order to retrace pathologic phenotypes, elucidate molecular mechanisms, and discover therapies using simple and reproducible techniques. In the past years, several human cell types have been utilized for these goals, including heterologous systems, cardiovascular and non-cardiovascular primary cells, and embryonic stem cells. The introduction of induced pluripotent stem cells and their differentiation potential brought new prospects for large-scale cardiovascular experiments, bypassing ethical concerns of embryonic stem cells and providing an advanced tool for disease modeling, diagnosis, and therapy. Each model has its advantages and disadvantages in terms of accessibility, maintenance, throughput, physiological relevance, recapitulation of the disease. A higher level of complexity in diseases modeling has been achieved with multicellular co-cultures. Furthermore, the important progresses reached by bioengineering during the last years, together with the opportunities given by pluripotent stem cells, have allowed the generation of increasingly advanced in vitro three-dimensional tissue-like constructs mimicking in vivo physiology. This review provides an overview of the main cell models used in cardiovascular research, highlighting the pros and cons of each, and describing examples of practical applications in disease modeling. View Full-Text
Keywords: human cell model; cardiovascular disease; disease modeling; heterologous system; primary cells; embryonic stem cells; human induced pluripotent stem cell; co-cultures; engineered 3D tissue human cell model; cardiovascular disease; disease modeling; heterologous system; primary cells; embryonic stem cells; human induced pluripotent stem cell; co-cultures; engineered 3D tissue
Show Figures

Figure 1

MDPI and ACS Style

Lippi, M.; Stadiotti, I.; Pompilio, G.; Sommariva, E. Human Cell Modeling for Cardiovascular Diseases. Int. J. Mol. Sci. 2020, 21, 6388. https://doi.org/10.3390/ijms21176388

AMA Style

Lippi M, Stadiotti I, Pompilio G, Sommariva E. Human Cell Modeling for Cardiovascular Diseases. International Journal of Molecular Sciences. 2020; 21(17):6388. https://doi.org/10.3390/ijms21176388

Chicago/Turabian Style

Lippi, Melania; Stadiotti, Ilaria; Pompilio, Giulio; Sommariva, Elena. 2020. "Human Cell Modeling for Cardiovascular Diseases" Int. J. Mol. Sci. 21, no. 17: 6388. https://doi.org/10.3390/ijms21176388

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop