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Open AccessArticle

DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides

1
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
3
Independent researcher, P.O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Miki-cho, Kita-gun, Kagawa-ken 761-0799, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6250; https://doi.org/10.3390/ijms21176250
Received: 7 August 2020 / Revised: 24 August 2020 / Accepted: 24 August 2020 / Published: 28 August 2020
(This article belongs to the Special Issue Orchid Biochemistry 2.0)
The acetylation or deacetylation of polysaccharides can influence their physical properties and biological activities. One main constituent of the edible medicinal orchid, Dendrobium officinale, is water-soluble polysaccharides (WSPs) with substituted O-acetyl groups. Both O-acetyl groups and WSPs show a similar trend in different organs, but the genes coding for enzymes that transfer acetyl groups to WSPs have not been identified. In this study, we report that REDUCED WALL ACETYLATION (RWA) proteins may act as acetyltransferases. Three DoRWA genes were identified, cloned, and sequenced. They were sensitive to abscisic acid (ABA), but there were no differences in germination rate and root length between wild type and 35S::DoRWA3 transgenic lines under ABA stress. Three DoRWA proteins were localized in the endoplasmic reticulum. DoRWA3 had relatively stronger transcript levels in organs where acetyl groups accumulated than DoRWA1 and DoRWA2, was co-expressed with polysaccharides synthetic genes, so it was considered as a candidate acetyltransferase gene. The level of acetylation of polysaccharides increased significantly in the seeds, leaves and stems of three 35S::DoRWA3 transgenic lines compared to wild type plants. These results indicate that DoRWA3 can transfer acetyl groups to polysaccharides and is a candidate protein to improve the biological activity of other edible and medicinal plants. View Full-Text
Keywords: acetyl groups; Dendrobium officinale; REDUCED WALL ACETYLATION; endoplasmic reticulum acetyl groups; Dendrobium officinale; REDUCED WALL ACETYLATION; endoplasmic reticulum
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MDPI and ACS Style

Si, C.; Teixeira da Silva, J.A.; He, C.; Yu, Z.; Zhao, C.; Wang, H.; Zhang, M.; Duan, J. DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides. Int. J. Mol. Sci. 2020, 21, 6250.

AMA Style

Si C, Teixeira da Silva JA, He C, Yu Z, Zhao C, Wang H, Zhang M, Duan J. DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides. International Journal of Molecular Sciences. 2020; 21(17):6250.

Chicago/Turabian Style

Si, Can; Teixeira da Silva, Jaime A.; He, Chunmei; Yu, Zhenming; Zhao, Conghui; Wang, Haobin; Zhang, Mingze; Duan, Jun. 2020. "DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides" Int. J. Mol. Sci. 21, no. 17: 6250.

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