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Impact of Nucleic Acid Sequencing on Viroid Biology

RNA Group/Groupe ARN, Département de Biochimie, Faculté de médecine des sciences de la santé, Pavillon de Recherche Appliquée au Cancer, Université de Sherbrooke, 3201 rue Jean Mignault, Sherbrooke, QC J1E 4K8, Canada
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Int. J. Mol. Sci. 2020, 21(15), 5532; https://doi.org/10.3390/ijms21155532
Received: 5 July 2020 / Revised: 29 July 2020 / Accepted: 30 July 2020 / Published: 1 August 2020
(This article belongs to the Special Issue Molecular Mechanisms of Viroids and Viroid Diseases)
The early 1970s marked two breakthroughs in the field of biology: (i) The development of nucleotide sequencing technology; and, (ii) the discovery of the viroids. The first DNA sequences were obtained by two-dimensional chromatography which was later replaced by sequencing using electrophoresis technique. The subsequent development of fluorescence-based sequencing method which made DNA sequencing not only easier, but many orders of magnitude faster. The knowledge of DNA sequences has become an indispensable tool for both basic and applied research. It has shed light biology of viroids, the highly structured, circular, single-stranded non-coding RNA molecules that infect numerous economically important plants. Our understanding of viroid molecular biology and biochemistry has been intimately associated with the evolution of nucleic acid sequencing technologies. With the development of the next-generation sequence method, viroid research exponentially progressed, notably in the areas of the molecular mechanisms of viroids and viroid diseases, viroid pathogenesis, viroid quasi-species, viroid adaptability, and viroid–host interactions, to name a few examples. In this review, the progress in the understanding of viroid biology in conjunction with the improvements in nucleotide sequencing technology is summarized. The future of viroid research with respect to the use of third-generation sequencing technology is also briefly envisaged. View Full-Text
Keywords: DNA sequencing; viroids; non-coding RNA; next-generation sequencing; third generation sequencing DNA sequencing; viroids; non-coding RNA; next-generation sequencing; third generation sequencing
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Adkar-Purushothama, C.R.; Perreault, J.-P. Impact of Nucleic Acid Sequencing on Viroid Biology. Int. J. Mol. Sci. 2020, 21, 5532.

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