Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating EMT-Activator Zeb1 and SK3 Channel Expression
Abstract
:1. Introduction
2. Results
2.1. Hypoxia Induces Zeb1 and SK3 Expression in Human Prostate Cancer Cells and Human Malignant Prostate Tissue
2.2. SK3 Channel Contributes to PCa Aggressiveness by Increasing Store-Operated Ca2+ Entry, Cell Migration, and Zeb1 Expression
2.3. LA and EPA Lipids Totally Block the Expression of Zeb1 and SK3 Induced by Hypoxia
3. Discussion
4. Materials and Methods
4.1. Human Tissue Slice Cultures
4.2. Immunohistochemistry
4.3. Cell Lines and Products
4.4. RNA Extraction and Quantitative Real-Time PCR
4.5. Cytosolic Calcium Measurements
4.6. Migration Assays
4.7. Transfection Assays
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
EMT | Epithelial-to-mesenchymal transition |
EPA | Eicosapentaenoic acid |
HIF-1α | Hypoxia-inducible factor 1α |
HRE | Hypoxia-response element |
FA | Fatty acid |
LA | Linoleic acid |
PA | Palmitic acid |
PCa | Prostate cancer |
SOCE | Store-operated Ca2+ entry |
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Bery, F.; Figiel, S.; Kouba, S.; Fontaine, D.; Guéguinou, M.; Potier-Cartereau, M.; Vandier, C.; Guibon, R.; Bruyère, F.; Fromont, G.; et al. Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating EMT-Activator Zeb1 and SK3 Channel Expression. Int. J. Mol. Sci. 2020, 21, 4786. https://doi.org/10.3390/ijms21134786
Bery F, Figiel S, Kouba S, Fontaine D, Guéguinou M, Potier-Cartereau M, Vandier C, Guibon R, Bruyère F, Fromont G, et al. Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating EMT-Activator Zeb1 and SK3 Channel Expression. International Journal of Molecular Sciences. 2020; 21(13):4786. https://doi.org/10.3390/ijms21134786
Chicago/Turabian StyleBery, Fanny, Sandy Figiel, Sana Kouba, Delphine Fontaine, Maxime Guéguinou, Marie Potier-Cartereau, Christophe Vandier, Roseline Guibon, Franck Bruyère, Gaëlle Fromont, and et al. 2020. "Hypoxia Promotes Prostate Cancer Aggressiveness by Upregulating EMT-Activator Zeb1 and SK3 Channel Expression" International Journal of Molecular Sciences 21, no. 13: 4786. https://doi.org/10.3390/ijms21134786