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Open AccessArticle

Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

1
Clinic of Stomatology, Faculty of Medicine, Masaryk University, Pekarska 664/53, 60200 Brno, Czech Republic
2
Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic
3
Clinic of Maxillofacial Surgery, University Hospital Brno, Jihlavska 20, 62500 Brno, Czech Republic
4
Clinic of Maxillofacial Surgery, Faculty of Medicine, Masaryk University, Jihlavska 20, 62500 Brno, Czech Republic
5
Clinic of Stomatology, St. Anne’s University Hospital, Pekarska 664/53, 60200 Brno, Czech Republic
6
Department of Burns and Plastic Surgery, Faculty of Medicine, Masaryk University, Jihlavska 20, 62500 Brno, Czech Republic
7
Department of Burns and Plastic Surgery, University Hospital Brno, Jihlavska 20, 62500 Brno, Czech Republic
8
Institute of Clinical and Experimental Dental Medicine, First Faculty of Medicine, Charles University, Karlovo nam. 554/32, 12808 Prague, Czech Republic
9
Institute of Clinical and Experimental Dental Medicine, General University Hospital, Karlovo nam. 554/32, 12808 Prague, Czech Republic
10
Department of Dentistry, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 50003 Hradec Kralove, Czech Republic
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4728; https://doi.org/10.3390/ijms21134728
Received: 4 June 2020 / Revised: 26 June 2020 / Accepted: 28 June 2020 / Published: 2 July 2020
(This article belongs to the Special Issue Molecular Mechanisms of Periodontal Disease)
Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms in IL-1 gene cluster, IL-6 and its receptor, IL-10, IL-17A, and IL-18 were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphism IL-10 −1087 A/G (rs1800896) and specific IL-10 haplotypes were associated with the development of the disease (p < 0.05, Pcorr > 0.05). Four bacterial species occurred more frequently in AgP than in controls (p < 0.01, Pcorr < 0.05). Elevated IL-10 levels were found in AgP patients, carriers of IL-10 −1087GG genotype, and PBMCs stimulated by periodontal bacteria (p < 0.05, Pcorr > 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression of IL-10 and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development. View Full-Text
Keywords: aggressive periodontitis; oral bacteria; inflammation; interleukin; polymorphism; genetic predisposition aggressive periodontitis; oral bacteria; inflammation; interleukin; polymorphism; genetic predisposition
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MDPI and ACS Style

Borilova Linhartova, P.; Danek, Z.; Deissova, T.; Hromcik, F.; Lipovy, B.; Szaraz, D.; Janos, J.; Fassmann, A.; Bartova, J.; Drizhal, I.; Izakovicova Holla, L. Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis. Int. J. Mol. Sci. 2020, 21, 4728. https://doi.org/10.3390/ijms21134728

AMA Style

Borilova Linhartova P, Danek Z, Deissova T, Hromcik F, Lipovy B, Szaraz D, Janos J, Fassmann A, Bartova J, Drizhal I, Izakovicova Holla L. Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis. International Journal of Molecular Sciences. 2020; 21(13):4728. https://doi.org/10.3390/ijms21134728

Chicago/Turabian Style

Borilova Linhartova, Petra; Danek, Zdenek; Deissova, Tereza; Hromcik, Filip; Lipovy, Bretislav; Szaraz, David; Janos, Julius; Fassmann, Antonin; Bartova, Jirina; Drizhal, Ivo; Izakovicova Holla, Lydie. 2020. "Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis" Int. J. Mol. Sci. 21, no. 13: 4728. https://doi.org/10.3390/ijms21134728

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