Next Article in Journal
Advances in the Development of Pharmacological Chaperones for the Mucopolysaccharidoses
Next Article in Special Issue
The Effect of Silver Nanoparticles on Antioxidant/Pro-Oxidant Balance in a Murine Model
Previous Article in Journal
Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique
Previous Article in Special Issue
Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma
Open AccessArticle

Molecular and Cellular Risk Assessment of Healthy Human Cells and Cancer Human Cells Exposed to Nanoparticles

1
Brazilian Nuclear Energy Commission, Nuclear Engineering Institute, Rio de Janeiro 21941906, Brazil
2
Laboratory of Cellular and Molecular Pharmacology, Department of Cell Biology, IBRAG, Universidade do Estado do Rio de Janeiro, Rio de Janeiro-RJ 21040900, Brazil
3
Department of Physics, Federal University of Maranhão, São Luis do Maranhão 65080-805, Brazil
4
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València. Camino de Vera s/n, 46022 Valencia, Spain
5
Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain
6
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Jordi Girona 18-26, ES 08034 Barcelona, Spain
7
Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro-RJ 21941-901, Brazil
8
Department of Bioengineering, Clemson University, Clemson, SC 29634, USA
9
Laboratory of Biology of Endothelial Cells and Angiogenesis (LabAngio), Department of Cell Biology, IBRAG, Universidade do Estado do Rio de Janeiro, Rio de Janeiro-RJ 20550-900, Brazil
10
Laboratory of Radiopharmacy and Nanoradiopharmaceuticals, Zona Oeste State University, Rio de Janeiro-RJ 23070-200, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 230; https://doi.org/10.3390/ijms21010230
Received: 15 October 2019 / Revised: 14 November 2019 / Accepted: 22 November 2019 / Published: 28 December 2019
(This article belongs to the Special Issue Nanomedicine, Nanopharmacy and Nanobiomaterials)
Nanodrugs have in recent years been a subject of great debate. In 2017 alone, almost 50 nanodrugs were approved for clinical use worldwide. Despite the advantages related to nanodrugs/nanomedicine, there is still a lack of information regarding the biological safety, as the real behavior of these nanodrugs in the body. In order to better understand these aspects, in this study, we evaluated the effect of polylactic acid (PLA) nanoparticles (NPs) and magnetic core mesoporous silica nanoparticles (MMSN), of 1000 nm and 50 nm, respectively, on human cells. In this direction we evaluated the cell cycle, cytochemistry, proliferation and tubulogenesis on tumor cells lines: from melanoma (MV3), breast cancer (MCF-7, MDA-MB-213), glioma (U373MG), prostate (PC3), gastric (AGS) and colon adenocarcinoma (HT-29) and non-tumor cell lines: from human melanocyte (NGM), fibroblast (FGH) and endothelial (HUVEC), respectively. The data showed that an acute exposure to both, polymeric nanoparticles or MMSN, did not show any relevant toxic effects on neither tumor cells nor non-tumor cells, suggesting that although nanodrugs may present unrevealed aspects, under acute exposition to human cells they are harmless. View Full-Text
Keywords: nanoparticles; nanomedicine; tumor cell; cell culture; primary cells nanoparticles; nanomedicine; tumor cell; cell culture; primary cells
Show Figures

Graphical abstract

MDPI and ACS Style

Helal-Neto, E.; de Barros, A.O.S.; Saldanha-Gama, R.; Brandão-Costa, R.; Alencar, L.M.R.; dos Santos, C.C.; Martínez-Máñez, R.; Ricci-Junior, E.; Alexis, F.; Morandi, V.; Barja-Fidalgo, C.; Santos-Oliveira, R. Molecular and Cellular Risk Assessment of Healthy Human Cells and Cancer Human Cells Exposed to Nanoparticles. Int. J. Mol. Sci. 2020, 21, 230.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop