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Int. J. Mol. Sci. 2019, 20(3), 792; https://doi.org/10.3390/ijms20030792

Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes in Populus simonii × P. nigra

1
Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, 368 Xuefu Road, Harbin 150086, China
2
Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin 150086, China
3
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
4
Crop Breeding Institute, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin 150086, China; [email protected]
5
Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, 103 Haping Road, Harbin 150040, China
6
College of Life Science, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 13 January 2019 / Revised: 6 February 2019 / Accepted: 11 February 2019 / Published: 12 February 2019
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Although phytohormones are known to be important signal molecules involved in wood formation, their roles are still largely unclear. Here, Populus simonii × P. nigra seedlings were treated with different concentrations of exogenous phytohormones, indole-3-acetic acid (IAA), gibberellin (GA3), and brassinosteroid (BR), and the effects of phytohormones on growth were investigated. Next, 27 genes with known roles in wood formation were selected for qPCR analysis to determine tissue-specificity and timing of responses to phytohormone treatments. Compared to the control, most IAA, GA3, and BR concentrations significantly increased seedling height. Meanwhile, IAA induced significant seedling stem diameter and cellulose content increases that peaked at 3 and 30 mg·L−1, respectively. Significant increase in cellulose content was also observed in seedlings treated with 100 mg·L−1 GA3. Neither stem diameter nor cellulose content of seedlings were affected by BR treatment significantly, although slight effects were observed. Anatomical measurements demonstrated improved xylem, but not phloem, development in IAA- and BR-treated seedlings. Most gene expression patterns induced by IAA, GA3, and BR differed among tissues. Many IAA response genes were also regulated by GA3, while BR-induced transcription was weaker and slower in Populus than for IAA and GA3. These results reveal the roles played by phytohormones in plant growth and lay the foundation for exploring molecular regulatory mechanisms of wood formation in Populus. View Full-Text
Keywords: Populus simonii × P. nigra; phytohormone; wood formation related gene; expression profiles; qPCR Populus simonii × P. nigra; phytohormone; wood formation related gene; expression profiles; qPCR
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Yuan, H.; Zhao, L.; Guo, W.; Yu, Y.; Tao, L.; Zhang, L.; Song, X.; Huang, W.; Cheng, L.; Chen, J.; Guan, F.; Wu, G.; Li, H. Exogenous Application of Phytohormones Promotes Growth and Regulates Expression of Wood Formation-Related Genes in Populus simonii × P. nigra. Int. J. Mol. Sci. 2019, 20, 792.

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