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Open AccessArticle

Resveratrol Treatment Enhances the Cellular Response to Leptin by Increasing OBRb Content in Palmitate-Induced Steatotic HepG2 Cells

1
Nutrigenomics Research Group, Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, 43007 Tarragona, Spain
2
Human Nutrition Unit, Department of Food and Drugs, University of Parma, 43125 Parma, Italy
3
Human Nutrition Unit, Department of Veterinary Medicine, University of Parma, 43125 Parma, Italy
4
School of Advanced Studies on Food and Nutrition, University of Parma, 43215 Parma, Italy
5
Microbiome Research Hub, University of Parma, 43125 Parma, Italy
6
Eurecat, Centre Tecnològic de Catalunya, Biotechnological Area, 43204 Reus, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6282; https://doi.org/10.3390/ijms20246282
Received: 20 November 2019 / Revised: 9 December 2019 / Accepted: 11 December 2019 / Published: 12 December 2019
The interaction of leptin with its hepatic longest receptor (OBRb) promotes the phosphorylation of signal transducer and activator of transcription-3 (STAT3), protecting the liver from lipid accumulation. However, leptin signalling is disrupted in hepatic steatosis, causing leptin resistance. One promising strategy to combat this problem is the use of bioactive compounds such as polyphenols. Since resveratrol (RSV) is a modulator of lipid homeostasis in the liver, we investigated whether treatment with different doses of RSV restores appropriate leptin action and fat accumulation in palmitate-induced steatotic human hepatoma (HepG2) cells. Both RSV metabolism and the expression of molecules implicated in leptin signalling were analysed. RSV at a 10 μM concentration was entirely metabolized to resveratrol-3-sulfate after 24 and counteracted leptin resistance by increasing the protein levels of OBRb. In addition, RSV downregulated the expression of lipogenic genes including fatty acid synthase (Fas) and stearoyl-CoA desaturase-1 (Scd1) without any significant change in Sirtuin-1 (SIRT1) enzymatic activity. These results demonstrate that RSV restored leptin sensitivity in a cellular model of hepatic steatosis in a SIRT1-independent manner. View Full-Text
Keywords: leptin resistance; lipid metabolism; non-alcoholic fatty liver disease (NAFLD); obesity; resveratrol metabolites; sirtuin 1 leptin resistance; lipid metabolism; non-alcoholic fatty liver disease (NAFLD); obesity; resveratrol metabolites; sirtuin 1
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Ardid-Ruiz, A.; Ibars, M.; Mena, P.; Del Rio, D.; Muguerza, B.; Arola, L.; Aragonès, G.; Suárez, M. Resveratrol Treatment Enhances the Cellular Response to Leptin by Increasing OBRb Content in Palmitate-Induced Steatotic HepG2 Cells. Int. J. Mol. Sci. 2019, 20, 6282.

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