Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation

Huang, Anqi; Honda, Yoshitomo; Li, Peiqi; Tanaka, Tomonari; Baba, Shunsuke

doi:10.3390/ijms20236042

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation

¹,

^2,*

¹,

^3,*

and

Shunsuke Baba

Department of Oral Implantology, Osaka Dental University, 1-5-17, Otemae, Chuo-ku, Osaka 540-0008, Japan

Institute of Dental Research, Osaka Dental University, 8-1, Kuzuhahanazonocho, Hirakata, Osaka 573-1121, Japan

Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(23), 6042; https://doi.org/10.3390/ijms20236042

Received: 19 September 2019 / Revised: 22 November 2019 / Accepted: 27 November 2019 / Published: 30 November 2019

(This article belongs to the Special Issue Functional Materials for Bone Regeneration: Biomaterials and Cells)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Matrix metalloproteinase (MMP)-2 and MMP-9 are well-known gelatinases that disrupt the extracellular matrix, including gelatin. However, the advantages of modulating MMP expression in gelatin-based materials for applications in bone regenerative medicine have not been fully clarified. In this study, we examined the effects of epigallocatechin gallate (EGCG), a major polyphenol catechin isolated from green tea, on MMP expression in gelatin sponges and its association with bone formation. Four gelatin sponges with or without EGCG were prepared and implanted into bone defects for up to 4 weeks. Histological and immunohistological staining were performed. Micro-computed tomography was used to estimate the bone-forming capacity of each sponge. Our results showed that EGCG integration attenuated MMP-2 (70.6%) and -9 expression (69.1%) in the 1 week group, increased residual gelatin (118.7%), and augmented bone formation (101.8%) in the 4 weeks group in critical-sized bone defects of rat calvaria compared with vacuum-heated gelatin sponges without EGCG. Moreover, vacuum-heated gelatin sponges with EGCG showed superior bone formation compared with other sponges. The results indicated that integration of EGCG in gelatin-based materials modulated the production and activity of MMP-2 and -9 in vivo, thereby enhancing bone-forming capacity. View Full-Text

Keywords: catechin; bone formation; anti-inflammatory reaction; matrix metalloproteinase; biomaterials

▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Share and Cite

MDPI and ACS Style

Huang, A.; Honda, Y.; Li, P.; Tanaka, T.; Baba, S. Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation. Int. J. Mol. Sci. 2019, 20, 6042. https://doi.org/10.3390/ijms20236042

AMA Style

Huang A, Honda Y, Li P, Tanaka T, Baba S. Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation. International Journal of Molecular Sciences. 2019; 20(23):6042. https://doi.org/10.3390/ijms20236042

Chicago/Turabian Style

Huang, Anqi, Yoshitomo Honda, Peiqi Li, Tomonari Tanaka, and Shunsuke Baba. 2019. "Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation" International Journal of Molecular Sciences 20, no. 23: 6042. https://doi.org/10.3390/ijms20236042

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Integration of Epigallocatechin Gallate in Gelatin Sponges Attenuates Matrix Metalloproteinase-Dependent Degradation and Increases Bone Formation

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI