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Open AccessArticle

Transcriptome Analysis of Sogatella furcifera (Homoptera: Delphacidae) in Response to Sulfoxaflor and Functional Verification of Resistance-Related P450 Genes

1
Biorational Pesticide Research Lab, Sichuan Agricultural University, Chengdu 611130, China
2
Sichuan Provincial Plant Protection Station, Department of Agriculture, Chengdu 610041, China
*
Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Int. J. Mol. Sci. 2019, 20(18), 4573; https://doi.org/10.3390/ijms20184573
Received: 18 August 2019 / Revised: 11 September 2019 / Accepted: 12 September 2019 / Published: 15 September 2019
(This article belongs to the Special Issue Cytochromes P450: Drug Metabolism, Bioactivation and Biodiversity 2.0)
The white-back planthopper (WBPH), Sogatella furcifera, is a major rice pest in China and in some other rice-growing countries of Asia. The extensive use of pesticides has resulted in severe resistance of S. furcifera to variety of chemical insecticides. Sulfoxaflor is a new diamide insecticide that acts on nicotinic acetylcholine receptors (nAChRs) in insects. The aim of this study was to explore the key genes related to the development of resistance to sulfoxaflor in S. furcifera and to verify their functions. Transcriptomes were compared between white-back planthoppers from a susceptible laboratory strain (Sus-Lab) and Sus-Lab screened with the sublethal LC25 dose of sulfoxaflor for six generations (SF-Sel). Two P450 genes (CYP6FD1 and CYP4FD2) and three transcription factors (NlE78sf, C2H2ZF1 and C2H2ZF3) with upregulated expression verified by qRT-PCR were detected in the Sus-Lab and SF-Sel strains. The functions of CYP6FD1 and CYP4FD2 were analyzed by RNA interference, and the relative normalized expressions of CYP6FD1 and CYP4FD2 in the SF-Sel population were lower than under dsGFP treatment after dsRNA injection. Moreover, the mortality rates of SF-Sel population treated with the LC50 concentration of sulfoxaflor after the injecting of dsRNA targeting CYP6FD1 and CYP4FD2 were significantly higher than in the dsGFP group from 72 h to 96 h (p < 0.05), and mortality in the CYP6FD1 knockdown group was clearly higher than that of the CYP4FD2 knockdown group. The interaction between the tertiary structures of CYP6FD1 and CYP4FD2 and sulfoxaflor was also predicted, and CYP6FD1 showed a stronger metabolic ability to process sulfoxaflor. Therefore, overexpression of CYP6FD1 and CYP4FD2 may be one of the primary factors in the development of sulfoxaflor resistance in S. furcifera. View Full-Text
Keywords: Sogatella furcifera; sulfoxaflor; transcriptome; cytochrome P450 monooxygenase; RNA interference Sogatella furcifera; sulfoxaflor; transcriptome; cytochrome P450 monooxygenase; RNA interference
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MDPI and ACS Style

Wang, X.-G.; Ruan, Y.-W.; Gong, C.-W.; Xiang, X.; Xu, X.; Zhang, Y.-M.; Shen, L.-T. Transcriptome Analysis of Sogatella furcifera (Homoptera: Delphacidae) in Response to Sulfoxaflor and Functional Verification of Resistance-Related P450 Genes. Int. J. Mol. Sci. 2019, 20, 4573. https://doi.org/10.3390/ijms20184573

AMA Style

Wang X-G, Ruan Y-W, Gong C-W, Xiang X, Xu X, Zhang Y-M, Shen L-T. Transcriptome Analysis of Sogatella furcifera (Homoptera: Delphacidae) in Response to Sulfoxaflor and Functional Verification of Resistance-Related P450 Genes. International Journal of Molecular Sciences. 2019; 20(18):4573. https://doi.org/10.3390/ijms20184573

Chicago/Turabian Style

Wang, Xue-Gui; Ruan, Yan-Wei; Gong, Chang-Wei; Xiang, Xin; Xu, Xiang; Zhang, Yu-Ming; Shen, Li-Tao. 2019. "Transcriptome Analysis of Sogatella furcifera (Homoptera: Delphacidae) in Response to Sulfoxaflor and Functional Verification of Resistance-Related P450 Genes" Int. J. Mol. Sci. 20, no. 18: 4573. https://doi.org/10.3390/ijms20184573

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