Next Article in Journal
psbE-psbL and ndhA Intron, the Promising Plastid DNA Barcode of Fagopyrum
Previous Article in Journal
The Intercalation of CORM-2 with Pharmaceutical Clay Montmorillonite (MMT) Aids for Therapeutic Carbon Monoxide Release
Previous Article in Special Issue
Norepinephrine Inhibits Synovial Adipose Stem Cell Chondrogenesis via α2a-Adrenoceptor-Mediated ERK1/2 Activation
Article Menu

Export Article

Open AccessArticle

Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head

1
AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland
2
IZKF Group Tissue Regeneration in Musculoskeletal Diseases, University Clinics Wuerzburg, 97070 Wuerzburg, Germany
3
Orthopedic Center for Musculoskeletal Research, University of Wuerzburg, 97076 Wuerzburg, Germany
4
Department of Orthopaedics, Erasmus MC, University Medical Center Rotterdam, 3015 Rotterdam, The Netherlands
5
Department of Oral and Maxillofacial Surgery, Erasmus MC, University Medical Center Rotterdam, 3015 Rotterdam, The Netherlands
6
Department of Trauma Surgery, Regensburg University Medical Center, 93053 Regensburg, Germany
7
Department of Orthopedic Surgery and Traumatology, Spine Unit, Inselspital, Bern University Hospital, 3010 Bern, Switzerland
8
Department of Orthopaedic Surgery, Washington University, St Louis, MO 63110, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3454; https://doi.org/10.3390/ijms20143454
Received: 13 May 2019 / Revised: 8 July 2019 / Accepted: 10 July 2019 / Published: 14 July 2019
  |  
PDF [6130 KB, uploaded 14 July 2019]
  |  

Abstract

(1) In vitro, bone marrow-derived stromal cells (BMSCs) demonstrate inter-donor phenotypic variability, which presents challenges for the development of regenerative therapies. Here, we investigated whether the frequency of putative BMSC sub-populations within the freshly isolated mononuclear cell fraction of bone marrow is phenotypically predictive for the in vitro derived stromal cell culture. (2) Vertebral body, iliac crest, and femoral head bone marrow were acquired from 33 patients (10 female and 23 male, age range 14–91). BMSC sub-populations were identified within freshly isolated mononuclear cell fractions based on cell-surface marker profiles. Stromal cells were expanded in monolayer on tissue culture plastic. Phenotypic assessment of in vitro derived cell cultures was performed by examining growth kinetics, chondrogenic, osteogenic, and adipogenic differentiation. (3) Gender, donor age, and anatomical site were neither predictive for the total yield nor the population doubling time of in vitro derived BMSC cultures. The abundance of freshly isolated progenitor sub-populations (CD45−CD34−CD73+, CD45−CD34−CD146+, NG2+CD146+) was not phenotypically predictive of derived stromal cell cultures in terms of growth kinetics nor plasticity. BMSCs derived from iliac crest and vertebral body bone marrow were more responsive to chondrogenic induction, forming superior cartilaginous tissue in vitro, compared to those isolated from femoral head. (4) The identification of discrete progenitor populations in bone marrow by current cell-surface marker profiling is not predictive for subsequently derived in vitro BMSC cultures. Overall, the iliac crest and the vertebral body offer a more reliable tissue source of stromal progenitor cells for cartilage repair strategies compared to femoral head. View Full-Text
Keywords: bone marrow stromal cells; MSC; pericytes; femoral head; vertebral body; iliac crest; chondrogenesis bone marrow stromal cells; MSC; pericytes; femoral head; vertebral body; iliac crest; chondrogenesis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Herrmann, M.; Hildebrand, M.; Menzel, U.; Fahy, N.; Alini, M.; Lang, S.; Benneker, L.; Verrier, S.; Stoddart, M.J.; Bara, J.J. Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head. Int. J. Mol. Sci. 2019, 20, 3454.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top