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Article

Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging

1
Department of Pharmacology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan
2
Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya 464-8650, Japan
3
Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai, Aichi 487-8501, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(11), 2825; https://doi.org/10.3390/ijms20112825
Received: 8 April 2019 / Revised: 28 May 2019 / Accepted: 7 June 2019 / Published: 10 June 2019
(This article belongs to the Special Issue Gangliosides: Modes of Action and Cell Fates)
Gangliosides are widely expressed in almost all tissues and cells and are also considered to be essential in the development and maintenance of various organs and tissues. However, little is known about their roles in bone metabolism. In this study, we investigated the effects of genetic deletion of ganglioside D3 (GD3) synthase, which is responsible for the generation of all b-series gangliosides, on bone metabolism. Although b-series gangliosides were not expressed in osteoblasts, these gangliosides were expressed in pre-osteoclasts. However, the expression of these gangliosides was decreased after induction of osteoclastogenesis by receptor activator of nuclear factor kappa-B ligand (RANKL). Three-dimensional micro-computed tomography (3D-μCT) analysis revealed that femoral cancellous bone mass in GD3 synthase-knockout (GD3S KO) mice was higher than that in wild type (WT) mice at the age of 40 weeks, although there were no differences in that between GD3S KO and WT mice at 15 weeks old. Whereas bone formation parameters (osteoblast numbers/bone surface and osteoblast surface/bone surface) in GD3S KO mice did not differ from WT mice, bone resorption parameters (osteoclast numbers/bone surface and osteoclast surface/bone surface) in GD3S KO mice became significantly lower than those in WT mice at 40 weeks of age. Collectively, this study demonstrates that deletion of GD3 synthase attenuates bone loss that emerges with aging. View Full-Text
Keywords: glycosphingolipids; gangliosides; glycosylation; bone metabolism glycosphingolipids; gangliosides; glycosylation; bone metabolism
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MDPI and ACS Style

Yo, S.; Hamamura, K.; Mishima, Y.; Hamajima, K.; Mori, H.; Furukawa, K.; Kondo, H.; Tanaka, K.; Sato, T.; Miyazawa, K.; Goto, S.; Togari, A. Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging. Int. J. Mol. Sci. 2019, 20, 2825. https://doi.org/10.3390/ijms20112825

AMA Style

Yo S, Hamamura K, Mishima Y, Hamajima K, Mori H, Furukawa K, Kondo H, Tanaka K, Sato T, Miyazawa K, Goto S, Togari A. Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging. International Journal of Molecular Sciences. 2019; 20(11):2825. https://doi.org/10.3390/ijms20112825

Chicago/Turabian Style

Yo, Shoyoku, Kazunori Hamamura, Yoshitaka Mishima, Kosuke Hamajima, Hironori Mori, Koichi Furukawa, Hisataka Kondo, Kenjiro Tanaka, Takuma Sato, Ken Miyazawa, Shigemi Goto, and Akifumi Togari. 2019. "Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging" International Journal of Molecular Sciences 20, no. 11: 2825. https://doi.org/10.3390/ijms20112825

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