Next Article in Journal
ERK Activity Dynamics during Zebrafish Embryonic Development
Previous Article in Journal
NFκB Inhibition Mitigates Serum Amyloid A-Induced Pro-Atherogenic Responses in Endothelial Cells and Leukocyte Adhesion and Adverse Changes to Endothelium Function in Isolated Aorta
Previous Article in Special Issue
Cholesterol Increases Lipid Binding Rate and Changes Binding Behavior of Bacillus thuringiensis Cytolytic Protein
Open AccessArticle

Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma

1
Department of the Medical Sciences and Human Oncology, University Aldo Moro, 70124 Bari, Italy
2
CNR Nanotec—Institute of Nanotechnology, 73100 Lecce, Italy
3
National Institute of Gastroenterology “S. de Bellis” Research Hospital, 70013 Castellana Grotte (Ba), Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(1), 108; https://doi.org/10.3390/ijms20010108
Received: 3 August 2018 / Revised: 20 September 2018 / Accepted: 16 October 2018 / Published: 28 December 2018
(This article belongs to the Special Issue Atomic Force Microscopy for Biological Applications)
The epithelial mesenchymal transition (EMT) is a physiological multistep process involving epithelial cells acquiring a mesenchymal-like phenotype. It is widely demonstrated that EMT is linked to tumor progression and metastasis. The transforming growth factor (TGF)-β pathways have been widely investigated, but its role in the hepatocarcinoma EMT is still unclear. While the biochemical pathways have been extensively studied, the alteration of biomechanical behavior correlated to cellular phenotype and motility is not yet fully understood. To better define the involvement of TGF-β1 in the metastatic progression process in different hepatocarcinoma cell lines (HepG2, PLC/PRF/5, HLE), we applied a systematic morphomechanical approach in order to investigate the physical and the structural characteristics. In addition, we evaluated the antitumor effect of LY2157299, a TGF-βR1 kinase inhibitor, from a biomechanical point of view, using Atomic Force and Confocal Microscopy. Our approach allows for validation of biological data, therefore it may be used in the future as a diagnostic tool to be combined with conventional biomolecular techniques. View Full-Text
Keywords: TGF-β1; epithelial to mesenchymal transition (EMT); atomic force microscopy (AFM) TGF-β1; epithelial to mesenchymal transition (EMT); atomic force microscopy (AFM)
Show Figures

Graphical abstract

MDPI and ACS Style

Cascione, M.; Leporatti, S.; Dituri, F.; Giannelli, G. Transforming Growth Factor-β Promotes Morphomechanical Effects Involved in Epithelial to Mesenchymal Transition in Living Hepatocellular Carcinoma. Int. J. Mol. Sci. 2019, 20, 108.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop