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ERK Activity Dynamics during Zebrafish Embryonic Development

Gene Regulation Research, Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara 630-0101, Japan
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Int. J. Mol. Sci. 2019, 20(1), 109; https://doi.org/10.3390/ijms20010109
Received: 22 November 2018 / Revised: 15 December 2018 / Accepted: 24 December 2018 / Published: 28 December 2018
(This article belongs to the Special Issue ERK Signaling Pathway in Diseases)
During vertebrate development, extracellular signal-regulated kinase (ERK) is activated by growth factors such as fibroblast growth factor (FGF), and it regulates the formation of tissues/organs including eyes, brains, somites, limbs, and inner ears. However, an experimental system to monitor ERK activity dynamics in the entire body of the vertebrate embryo is lacking. We recently studied ERK activity dynamics in the pre-somitic mesoderm of living zebrafish embryos injected with mRNAs encoding a Förster resonance energy transfer (FRET)-based ERK biosensor. In this study, transgenic zebrafish stably and ubiquitously expressing the ERK biosensor were generated to monitor ERK activity dynamics throughout embryonic development. The system allowed the identification of ERK activation domains in embryos from the late blastula to the late segmentation stage, consistent with immunostaining patterns obtained using anti-phosphorylated ERK antibody. A spatiotemporal map of ERK activity in the entire body during zebrafish embryogenesis was generated, and previously unidentified activation dynamics and ERK domains were identified. The proposed system is the first reported method to monitor ERK activity dynamics during vertebrate embryogenesis, providing insight into the role of ERK activity in normal and abnormal development in living vertebrate embryos. View Full-Text
Keywords: mitogen-activated protein kinase (MAPK); biosensor; signal activity; vertebrate development mitogen-activated protein kinase (MAPK); biosensor; signal activity; vertebrate development
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MDPI and ACS Style

Wong, K.-L.; Akiyama, R.; Bessho, Y.; Matsui, T. ERK Activity Dynamics during Zebrafish Embryonic Development. Int. J. Mol. Sci. 2019, 20, 109. https://doi.org/10.3390/ijms20010109

AMA Style

Wong K-L, Akiyama R, Bessho Y, Matsui T. ERK Activity Dynamics during Zebrafish Embryonic Development. International Journal of Molecular Sciences. 2019; 20(1):109. https://doi.org/10.3390/ijms20010109

Chicago/Turabian Style

Wong, Kah-Loon, Ryutaro Akiyama, Yasumasa Bessho, and Takaaki Matsui. 2019. "ERK Activity Dynamics during Zebrafish Embryonic Development" International Journal of Molecular Sciences 20, no. 1: 109. https://doi.org/10.3390/ijms20010109

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