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Article

Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)

by 1,2,3, 1,2, 1,2, 3, 3, 1,2, 1,2,4, 1,4, 1,4, 1,4, 1, 3, 1,4,* and 1,3,4,*
1
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
2
School of Life Sciences, University of Science and Technology of China, Hefei 230026, China
3
The Sericultural Research Institute, Anhui Academy of Agricultural Science, Hefei 230061, China
4
Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(9), 2799; https://doi.org/10.3390/ijms19092799
Received: 23 August 2018 / Revised: 14 September 2018 / Accepted: 14 September 2018 / Published: 17 September 2018
(This article belongs to the Section Biochemistry)
In this study, a bacterial strain exhibiting high selenite (Na2SeO3) tolerance and reduction capacity was isolated from the gut of Monochamus alternatus larvae and identified as Alcaligenes faecalis Se03. The isolate exhibited extreme tolerance to selenite (up to 120 mM) when grown aerobically. In the liquid culture medium, it was capable of reducing nearly 100% of 1.0 and 5.0 mM Na2SeO3 within 24 and 42 h, respectively, leading to the formation of selenium nanoparticles (SeNPs). Electron microscopy and energy dispersive X-ray analysis demonstrated that A. faecalis Se03 produced spherical electron-dense SeNPs with an average hydrodynamic diameter of 273.8 ± 16.9 nm, localized mainly in the extracellular space. In vitro selenite reduction activity and real-time PCR indicated that proteins such as sulfite reductase and thioredoxin reductase present in the cytoplasm were likely to be involved in selenite reduction and the SeNPs synthesis process in the presence of NADPH or NADH as electron donors. Finally, using Fourier transform infrared spectroscopy, protein and carbohydrate residues were detected on the surface of the biogenic SeNPs. Based on these observations, A. faecalis Se03 has the potential to be an eco-friendly candidate for the bioremediation of selenium-contaminated soil/water and a bacterial catalyst for the biogenesis of SeNPs. View Full-Text
Keywords: selenite reduction; Alcaligenes faecalis; biogenic selenium nanoparticles; electron microscopy analysis; real-time PCR selenite reduction; Alcaligenes faecalis; biogenic selenium nanoparticles; electron microscopy analysis; real-time PCR
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MDPI and ACS Style

Wang, Y.; Shu, X.; Zhou, Q.; Fan, T.; Wang, T.; Chen, X.; Li, M.; Ma, Y.; Ni, J.; Hou, J.; Zhao, W.; Li, R.; Huang, S.; Wu, L. Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae). Int. J. Mol. Sci. 2018, 19, 2799. https://doi.org/10.3390/ijms19092799

AMA Style

Wang Y, Shu X, Zhou Q, Fan T, Wang T, Chen X, Li M, Ma Y, Ni J, Hou J, Zhao W, Li R, Huang S, Wu L. Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae). International Journal of Molecular Sciences. 2018; 19(9):2799. https://doi.org/10.3390/ijms19092799

Chicago/Turabian Style

Wang, Yuting, Xian Shu, Qing Zhou, Tao Fan, Taichu Wang, Xue Chen, Minghao Li, Yuhan Ma, Jun Ni, Jinyan Hou, Weiwei Zhao, Ruixue Li, Shengwei Huang, and Lifang Wu. 2018. "Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)" International Journal of Molecular Sciences 19, no. 9: 2799. https://doi.org/10.3390/ijms19092799

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