Next Article in Journal
Treatment, Therapy and Management of Metabolic Epilepsy: A Systematic Review
Next Article in Special Issue
Stem Cells Therapy for Spinal Cord Injury
Previous Article in Journal
Characterization of Multiple Cytokine Combinations and TGF-β on Differentiation and Functions of Myeloid-Derived Suppressor Cells
Previous Article in Special Issue
Stress-Activated Protein Kinases in Spinal Cord Injury: Focus on Roles of p38
Correction published on 2 July 2018, see Int. J. Mol. Sci. 2018, 19(7), 1942.
Open AccessArticle

Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury

1
Institute of Neuroimmunology, Slovak Academy of Sciences, Dúbravská cesta 9, 845 10 Bratislava, Slovakia
2
Department of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
3
Université de Lille, Inserm, U-1192—Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France
4
Department of Epizootology and Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 870; https://doi.org/10.3390/ijms19030870
Received: 15 February 2018 / Revised: 4 March 2018 / Accepted: 9 March 2018 / Published: 15 March 2018
(This article belongs to the Special Issue Therapeutic Strategies to Spinal Cord Injury)
It was recently shown that the conditioned medium (CM) of mesenchymal stem cells can enhance viability of neural and glial cell populations. In the present study, we have investigated a cell-free approach via CM from rat bone marrow stromal cells (MScCM) applied intrathecally (IT) for spinal cord injury (SCI) recovery in adult rats. Functional in vitro test on dorsal root ganglion (DRG) primary cultures confirmed biological properties of collected MScCM for production of neurosphere-like structures and axon outgrowth. Afterwards, rats underwent SCI and were treated with IT delivery of MScCM or vehicle at postsurgical Days 1, 5, 9, and 13, and left to survive 10 weeks. Rats that received MScCM showed significantly higher motor function recovery, increase in spared spinal cord tissue, enhanced GAP-43 expression and attenuated inflammation in comparison with vehicle-treated rats. Spared tissue around the lesion site was infiltrated with GAP-43-labeled axons at four weeks that gradually decreased at 10 weeks. Finally, a cytokine array performed on spinal cord extracts after MScCM treatment revealed decreased levels of IL-2, IL-6 and TNFα when compared to vehicle group. In conclusion, our results suggest that molecular cocktail found in MScCM is favorable for final neuroregeneration after SCI. View Full-Text
Keywords: spinal cord injury; inflammation; regeneration; stem cells-derived conditioned media spinal cord injury; inflammation; regeneration; stem cells-derived conditioned media
Show Figures

Graphical abstract

MDPI and ACS Style

Cizkova, D.; Cubinkova, V.; Smolek, T.; Murgoci, A.-N.; Danko, J.; Vdoviakova, K.; Humenik, F.; Cizek, M.; Quanico, J.; Fournier, I.; Salzet, M. Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury. Int. J. Mol. Sci. 2018, 19, 870.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop