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A Comparison of the Effects of Benzalkonium Chloride on Ocular Surfaces between C57BL/6 and BALB/c Mice

1
Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-Sen University, Guangzhou 510060, China
2
Department of Ophthalmology, Hubei University of Science and Technology, Xianning 437100, China
*
Author to whom correspondence should be addressed.
Academic Editor: Igor A. Butovich
Int. J. Mol. Sci. 2017, 18(3), 509; https://doi.org/10.3390/ijms18030509
Received: 1 January 2017 / Revised: 13 February 2017 / Accepted: 23 February 2017 / Published: 26 February 2017
(This article belongs to the Special Issue Dry Eye and Ocular Surface Disorders)
Models of benzalkonium chloride (BAC)-induced ocular disruption have been created and are widely used in various animals. This study aimed to compare the effects of BAC on the ocular surfaces of C57BL/6 and BALB/c mice. C57BL/6 and BALB/c mice were treated separately with BAC eye-drops at different concentrations. Eyes were evaluated by scoring epithelial disruption, corneal opacity and neovascularization in vivo, and by histological assays with hematoxylin/eosin (H/E) and periodic acid-Schiff stainings and by determining the expression of inflammatory factors in vitro on Days 7 and 14. The in vivo corneal epithelial disruption, corneal edema/opacity and neovascularization, which were in accordance with the results of the H/E staining and peaked at Day 7, were observed in a dose-dependent manner in the BAC-treated mice, with more severe signs in the C57BL/6 mice than the BALB/c mice. The loss of conjunctival goblet cells in the conjunctivas and the increasing expression of monocyte chemoattractant protein 1 (MCP-1), growth-regulated protein alpha (GROa) and macrophage inflammatory protein-1 alpha (MIP-1a) in the corneas were found in a dose-dependent manner in both strains of mice. Topical application of BAC can dramatically disrupt the ocular surfaces of C57BL/6 and BALB/c mice, and the disruptions were much more severe in the C57BL/6 mice that received high doses of BAC. View Full-Text
Keywords: benzalkonium chloride; cornea; conjunctiva; mouse; C57BL/6; BALB/c benzalkonium chloride; cornea; conjunctiva; mouse; C57BL/6; BALB/c
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MDPI and ACS Style

Yang, Q.; Zhang, Y.; Liu, X.; Wang, N.; Song, Z.; Wu, K. A Comparison of the Effects of Benzalkonium Chloride on Ocular Surfaces between C57BL/6 and BALB/c Mice. Int. J. Mol. Sci. 2017, 18, 509.

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