Next Article in Journal
Affinity Purification of Binding miRNAs for Messenger RNA Fused with a Common Tag
Next Article in Special Issue
Resveratrol Induces the Expression of Interleukin-10 and Brain-Derived Neurotrophic Factor in BV2 Microglia under Hypoxia
Previous Article in Journal
The Effect of Fiber Bleaching Treatment on the Properties of Poly(lactic acid)/Oil Palm Empty Fruit Bunch Fiber Composites
Previous Article in Special Issue
Effects of (−)-Gallocatechin-3-Gallate on Tetrodotoxin-Resistant Voltage-Gated Sodium Channels in Rat Dorsal Root Ganglion Neurons
Open AccessArticle

Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1

1
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon 305-764, Korea
2
Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea
3
Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia
4
Department of Plant Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung-si, Gangwon-do 210-702, Korea
5
Visiting Professor Program (VPP), King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2014, 15(8), 14743-14752; https://doi.org/10.3390/ijms150814743
Received: 30 May 2014 / Revised: 17 July 2014 / Accepted: 22 July 2014 / Published: 22 August 2014
(This article belongs to the Special Issue Pharmaceuticals and Nutraceuticals by Molecular Farming)
To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines showing over-expression of AtPAP1 were generated. In order to investigate the regulation of AtPAP1 on the activities of CGA biosynthetic genes, the expression levels of seven P. grandiflorum CGA biosynthetic genes were analyzed in the hairy root line that had the greatest accumulation of AtPAP1 transcript, OxPAP1-1. The introduction of AtPAP1 increased the mRNA levels of all examined CGA biosynthetic genes and resulted in a 900% up-regulation of CGA accumulation in OxPAP1-1 hairy roots relative to controls. This suggests that P. grandiflorum hairy roots that over-express the AtPAP1 gene are a potential alternative source of roots for the production of CGA. View Full-Text
Keywords: Agrobacterium rhizogenes; chlorogenic acid; gene over-expression; hairy root; Platycodon grandiflorum; transcription factor; production of anthocyanin pigment 1 Agrobacterium rhizogenes; chlorogenic acid; gene over-expression; hairy root; Platycodon grandiflorum; transcription factor; production of anthocyanin pigment 1
Show Figures

Figure 1

MDPI and ACS Style

Tuan, P.A.; Kwon, D.Y.; Lee, S.; Arasu, M.V.; Al-Dhabi, N.A.; Park, N.I.; Park, S.U. Enhancement of Chlorogenic Acid Production in Hairy Roots of Platycodon grandiflorum by Over-Expression of An Arabidopsis thaliana Transcription Factor AtPAP1. Int. J. Mol. Sci. 2014, 15, 14743-14752.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop