Antioxidant and ACE-Inhibitory Activity of Protein Hydrolysates Produced from Atlantic Sea Cucumber (Cucumaria frondosa)
Abstract
1. Introduction
2. Results and Discussion
2.1. Hydroxyl Radical Scavenging Activity
2.2. Reducing Power
2.3. Inhibition of Peroxyl and Hydroxyl Radical-Induced Supercoiled DNA Strand Scission
2.4. Inhibition of Cupric Ion-Induced Human Low-Density Lipoprotein (LDL) Peroxidation
2.5. Angiotensin I Converting Enzyme (ACE) Inhibitory Activity
3. Materials and Methods
3.1. Preparation of Protein Hydrolysates
3.2. Hydroxyl Radical Scavenging Activity
(EPR signal intensity for the control − EPR signal intensity for the sample)/EPR signal intensity for the control × 100
3.3. Reducing Power
3.4. Inhibition of Peroxyl and Hydroxyl Radical-Induced Supercoiled DNA Strand Scission
3.5. Inhibition of Cupric Ion-Induced Human Low-Density Lipoprotein (LDL) Peroxidation
3.6. Angiotensin I Converting Enzyme (ACE) Inhibitory Activity
3.7. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Sample | DNA Scission Inhibition (%) | |
---|---|---|
Hydroxyl Radical | Peroxyl Radical | |
BWA | 29.61 ± 0.75 c | 56.11 ± 1.24 c |
BWF | 72.18 ± 2.95 ab | 69.45 ± 3.90 bc |
BWC | 69.09 ± 1.18 b | 71.19 ± 2.89 b |
BW(C+F) | 68.61 ± 2.01 b | 72.51 ± 2.63 b |
BW(A+F) | 77.22 ± 2.59 a | 81.85 ± 0.18 a |
FLA | 38.59 ± 2.27 d | 57.41 ± 3.53 d |
FLF | 37.64 ± 2.69 d | 63.06 ± 2.47 c |
FLC | 69.24 ± 0.51 b | 72.67 ± 1.32 b |
FL(C+F) | 72.58 ± 0.65 ab | 77.31 ± 0.25 ab |
FL(A+F) | 76.94 ± 1.09 a | 79.79 ± 1.36 a |
INA | 35.77 ± 1.55 c | 57.14 ± 2.64 a |
INF | 44.48 ± 2.38 b | 32.90 ± 1.10 c |
INC | 58.49 ± 2.50 a | 61.53 ± 3.16 a |
IN(C+F) | 46.71 ± 3.27 b | 53. 67 ± 1.47 ab |
IN(A+F) | 49.49 ± 0.95 b | 47.28 ± 2.02 b |
Carnosine | 77.32 ± 1.42 | 83.01 ± 0.56 |
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Senadheera, T.R.L.; Hossain, A.; Dave, D.; Shahidi, F. Antioxidant and ACE-Inhibitory Activity of Protein Hydrolysates Produced from Atlantic Sea Cucumber (Cucumaria frondosa). Molecules 2023, 28, 5263. https://doi.org/10.3390/molecules28135263
Senadheera TRL, Hossain A, Dave D, Shahidi F. Antioxidant and ACE-Inhibitory Activity of Protein Hydrolysates Produced from Atlantic Sea Cucumber (Cucumaria frondosa). Molecules. 2023; 28(13):5263. https://doi.org/10.3390/molecules28135263
Chicago/Turabian StyleSenadheera, Tharindu R. L., Abul Hossain, Deepika Dave, and Fereidoon Shahidi. 2023. "Antioxidant and ACE-Inhibitory Activity of Protein Hydrolysates Produced from Atlantic Sea Cucumber (Cucumaria frondosa)" Molecules 28, no. 13: 5263. https://doi.org/10.3390/molecules28135263
APA StyleSenadheera, T. R. L., Hossain, A., Dave, D., & Shahidi, F. (2023). Antioxidant and ACE-Inhibitory Activity of Protein Hydrolysates Produced from Atlantic Sea Cucumber (Cucumaria frondosa). Molecules, 28(13), 5263. https://doi.org/10.3390/molecules28135263