Next Article in Journal
Discovery of Novel Pleuromutilin Derivatives as Potent Antibacterial Agents for the Treatment of MRSA Infection
Previous Article in Journal
Preliminary Study of Resistance Mechanism of Botrytis cinerea to SYAUP-CN-26
Article

Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity

by 1, 1, 1, 1,2,* and 1,2,*
1
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110006, China
2
Liaoning Provincial Key Laboratory of TCM Resources Conservation and Development, Shenyang Pharmaceutical University, Shenyang 110006, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Satyajit Sarker
Molecules 2022, 27(3), 938; https://doi.org/10.3390/molecules27030938
Received: 29 December 2021 / Revised: 26 January 2022 / Accepted: 27 January 2022 / Published: 29 January 2022
The cause of liver damage by using black cohosh preparation has been concerned but remains unclear. After a preliminary investigation, the black cohosh medicinal materials sold in the market were adulterated with Asian cohosh (Cimicifuga) without removing the fibrous roots. The safety of Cimicifuga rhizome and fibrous roots is unknown and has not been reported. Therefore, in this paper, the rhizome and fibrous roots of Cimicifuga dahurica (Turcz.) Maxim (C. dahurica) were completely separated, extracted with 70% ethanol, and freeze-dried to obtain crude rhizome extract (RC) and fibrous roots extract (FRC). UHPLC-Q-TOF-MS was used to identify 39 compounds in the rhizome and fibrous roots of Cimicifuga, mainly saponins and phenolic acids. In the L-02 cytotoxicity experiment, the IC50 of fibrous roots (1.26 mg/mL) was slightly lower than that of rhizomes (1.417 mg/mL). In the 90-day sub-chronic toxicity study, the FRC group significantly increased the level of white blood cells, ALP, ALT, AST, BILI and CHOL (p < 0.05); large area of granular degeneration and balloon degeneration occurred in liver tissue; and the expression of p-NF-kB in the nucleus increased in a dose-dependent manner. Overall, Fibrous roots of Cimicifuga are at risk of hepatotoxicity and should be strictly controlled and removed during the processing. View Full-Text
Keywords: hepatotoxicity; fibrous roots; rhizome; Cimicifuga dahurica (Turcz.) Maxim hepatotoxicity; fibrous roots; rhizome; Cimicifuga dahurica (Turcz.) Maxim
Show Figures

Graphical abstract

MDPI and ACS Style

Yu, Y.; Tan, J.; Nie, J.; Lv, C.; Lu, J. Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity. Molecules 2022, 27, 938. https://doi.org/10.3390/molecules27030938

AMA Style

Yu Y, Tan J, Nie J, Lv C, Lu J. Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity. Molecules. 2022; 27(3):938. https://doi.org/10.3390/molecules27030938

Chicago/Turabian Style

Yu, Yang, Jialiang Tan, Jianing Nie, Chongning Lv, and Jincai Lu. 2022. "Fibrous Roots of Cimicifuga Are at Risk of Hepatotoxicity" Molecules 27, no. 3: 938. https://doi.org/10.3390/molecules27030938

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop