Preliminary Study of Resistance Mechanism of Botrytis cinerea to SYAUP-CN-26
Abstract
:1. Introduction
2. Results
2.1. Generation of B. cinerea Mutant Resistance to SYAUP-CN-26
2.2. Effect of SYAUP-CN-26 on Cell Membrane
2.3. Measurement of Relative Electric Conductivity
2.4. Effect of SYAUP-CN-26 on the Triglyceride Synthesis (TG) of B. cinerea
2.5. Detection of Histidine Kinase (HK) Activity
2.6. Sequence Variation in the Bos1 Gene
3. Discussion
4. Materials and Methods
4.1. Fungal Isolates, Chemicals, and Culture Media
4.2. Generation of Resistant Mutants of B. cinerea to SYAUP-CN-26
4.3. Level and Stability of Resistance of Mutants to SYAUP-CN-26
4.4. Effect of SYAUP-CN-26 on Cell Membrane Structure
4.5. Measurement of Relative Electric Conductivity
4.6. Effect of SYAUP-CN-26 on Triglyceride Synthesis (TG)
4.7. Effect of SYAUP-CN-26 on Histidine Kinase Activity
4.8. Sequences of the Bos1 Gene in Sensitive and Resistant Strains
4.9. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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EC50 (µg/mL) | RF | FSC | |||
---|---|---|---|---|---|
1st | 10th | 1st | 10th | ||
5055 | 1.6047 | 1.6561 | -- | -- | |
5055-R4 | 111.0821 | 115.7630 | 69.2230 | 69.9010 | 1.0098 |
AF396827.2 | 329 | ******* | 348 | ****** | 368 | 369 | 370 | 371 | 372 | 373 |
CAA | ******* | CCG | ****** | GTC | CAG | CGC | ATG | TGG | AAC | |
Q | ******* | P | ****** | D | Q | R | M | W | N | |
5505 | CAA | ****** | CCG | ****** | GTC | CAG | CGC | ATG | TGG | AAC |
Q | ****** | P | ****** | D | Q | R | M | W | N | |
5505-R1 | CAA | ****** | CCG | ****** | GTC | CAG | CGC | ATG | TGG | AAC |
Q | ****** | P | ****** | D | Q | R | M | W | N | |
5505-R2 | CAA | ****** | CTG | ****** | GTC | CAG | CGC | ATG | TGG | AAC |
Q | ****** | L | ****** | D | Q | R | M | W | N | |
5505-R3 | CAA | ****** | CTG | ****** | GTC | CAG | CGC | ATG | TGG | AAC |
Q | ****** | L | ****** | D | Q | R | M | W | N | |
5505-R4 | CAA | ****** | CTG | ****** | GTC | CAG | CGC | ATG | TGG | AAC |
Q | ****** | L | ****** | D | Q | R | M | W | N | |
5505-R5 | CAA | ****** | CTG | ****** | GTC | CAG | CGC | ATG | TGG | AAC |
Q | ****** | L | ****** | D | Q | R | M | W | N |
Primers | Sequences | Annealing Temperature | Gene Sizes |
---|---|---|---|
F1: | AACGCGTAGCAGCTCTCGAA | 62 | 1496 |
R1 | ATGGTAAGATGGGTGGCCAA | ||
F2 | GCGGGTGAAATACTCATACT | 60 | 992 |
R2 | ACGCTATCAAGTTCACAGAG | ||
F3 | AACCCAAAGTCCCAATCCCAA | 58 | 1948 |
R3 | TTCTCGGTGGACAAGCCAA | ||
F4 | TTACCGCGTAGATAGCTCAGTT | 60 | 1620 |
R4 | GCCGAAGTTCGATGATAT |
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Wang, K.; Zhang, H.; Zhu, W.; Peng, J.; Li, X.; Wang, Y.; Qi, Z. Preliminary Study of Resistance Mechanism of Botrytis cinerea to SYAUP-CN-26. Molecules 2022, 27, 936. https://doi.org/10.3390/molecules27030936
Wang K, Zhang H, Zhu W, Peng J, Li X, Wang Y, Qi Z. Preliminary Study of Resistance Mechanism of Botrytis cinerea to SYAUP-CN-26. Molecules. 2022; 27(3):936. https://doi.org/10.3390/molecules27030936
Chicago/Turabian StyleWang, Kai, Huazhong Zhang, Wanying Zhu, Jingnan Peng, Xinghai Li, Yingzi Wang, and Zhiqiu Qi. 2022. "Preliminary Study of Resistance Mechanism of Botrytis cinerea to SYAUP-CN-26" Molecules 27, no. 3: 936. https://doi.org/10.3390/molecules27030936