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Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
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Academic Editor: Roberto Fernandez-Lafuente
Molecules 2021, 26(21), 6564; https://doi.org/10.3390/molecules26216564
Received: 29 September 2021 / Revised: 27 October 2021 / Accepted: 28 October 2021 / Published: 29 October 2021
Background: Tumor-targeting bacteriophages can be used as a versatile new platform for the delivery of diagnostic imaging agents and therapeutic cargo. This became possible due to the development of viral capsid modification method. Earlier in our laboratory and using phage display technology, phages to malignant breast cancer cells MDA-MB 231 were obtained. The goal of this study was the optimization of phage modification and the assessment of the effect of the latter on the efficiency of phage particle penetration into MDA-MB 231 cells. Methods: In this work, we used several methods, such as chemical phage modification using FAM-NHS ester, spectrophotometry, phage amplification, sequencing, phage titration, flow cytometry, and confocal microscopy. Results: We performed chemical phage modification using different concentrations of FAM-NHS dye (0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM). It was shown that with an increase of the modification degree, the phage titer decreases. The maximum modification coefficient of the phage envelope with the FAM–NHS dye was observed with 4 mM modifying agent and had approximately 804,2 FAM molecules per phage. Through the immunofluorescence staining and flow cytometry methods, it was shown that the modified bacteriophage retains the ability to internalize into MDA-MB-231 cells. The estimation of the number of phages that could have penetrated into one tumor cell was conducted. Conclusions: Optimizing the conditions for phage modification can be an effective strategy for producing tumor-targeting diagnostic and therapeutic agents, i.e., theranostic drugs. View Full-Text
Keywords: MDA-MB 231 cells; tumor-targeting bacteriophages; chemical modification of bacteriophages; FAM–NHS; theranostic drugs MDA-MB 231 cells; tumor-targeting bacteriophages; chemical modification of bacteriophages; FAM–NHS; theranostic drugs
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MDPI and ACS Style

Dymova, M.A.; Utkin, Y.A.; Dmitrieva, M.D.; Kuligina, E.V.; Richter, V.A. Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications. Molecules 2021, 26, 6564. https://doi.org/10.3390/molecules26216564

AMA Style

Dymova MA, Utkin YA, Dmitrieva MD, Kuligina EV, Richter VA. Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications. Molecules. 2021; 26(21):6564. https://doi.org/10.3390/molecules26216564

Chicago/Turabian Style

Dymova, Maya A., Yaroslav A. Utkin, Maria D. Dmitrieva, Elena V. Kuligina, and Vladimir A. Richter. 2021. "Modification of a Tumor-Targeting Bacteriophage for Potential Diagnostic Applications" Molecules 26, no. 21: 6564. https://doi.org/10.3390/molecules26216564

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