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A Molecular Probe with Both Chromogenic and Fluorescent Units for Detecting Serine Proteases

Department of Biomedical Sciences and Informatics, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto 610-0394, Japan
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Author to whom correspondence should be addressed.
Academic Editor: Sheshanath Bhosale
Molecules 2021, 26(2), 482; https://doi.org/10.3390/molecules26020482
Received: 7 December 2020 / Revised: 15 January 2021 / Accepted: 15 January 2021 / Published: 18 January 2021
(This article belongs to the Section Organic Chemistry)
A molecular probe with l-phenylalanine p-nitroanilide and l-lysin 4-methylcoumaryl-7-amide, in which these amino acid derivatives are connected through a succinic-acid spacer, was prepared. Trypsin and papain were detected by blue-fluorescence emission of generated 7-amino-4-methylcoumarin (AMC). α-Chymotrypsin and nattokinase were detected from both the blue-fluorescence emission of AMC and the UV absorbance of p-nitroaniline. In addition, different time courses of p-nitroaniline and AMC were observed between the reaction of P1 with α-chymotrypsin and that with nattokinase. In the case of nattokinase, both the fluorescence emission and UV absorbance slowly increased. In contrast, the increasing UV absorbance was saturated at the early stage of the reaction of the present probe with chymotrypsin, whereas the fluorescence emission continuously increased in the following stages. View Full-Text
Keywords: molecular fluorescence probe; chromogenic molecular probe; protease; analysis molecular fluorescence probe; chromogenic molecular probe; protease; analysis
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MDPI and ACS Style

Ishida, K.; Nakamura, Y.; Ohta, T.; Oe, Y. A Molecular Probe with Both Chromogenic and Fluorescent Units for Detecting Serine Proteases. Molecules 2021, 26, 482. https://doi.org/10.3390/molecules26020482

AMA Style

Ishida K, Nakamura Y, Ohta T, Oe Y. A Molecular Probe with Both Chromogenic and Fluorescent Units for Detecting Serine Proteases. Molecules. 2021; 26(2):482. https://doi.org/10.3390/molecules26020482

Chicago/Turabian Style

Ishida, Kirara, Yushi Nakamura, Tetsuo Ohta, and Yohei Oe. 2021. "A Molecular Probe with Both Chromogenic and Fluorescent Units for Detecting Serine Proteases" Molecules 26, no. 2: 482. https://doi.org/10.3390/molecules26020482

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