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Open AccessArticle

High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression

by Jiasong Chang 1,2,3,4, Xiaoxu Chen 1,2,3,4, Ruolin Wang 1,2,3,4, Run Shi 1,2,3,4, Xiaogang Wang 1,2,3,4, Wei Lu 1,2,3,4, Sanyuan Ma 1,2,3,4,* and Qingyou Xia 1,2,3,4,*
1
Biological Science Research Center, Southwest University, Chongqing 400716, China
2
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
3
Chongqing Key Laboratory of Sericulture, Southwest University, Chongqing 400716, China
4
Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400716, China
*
Authors to whom correspondence should be addressed.
Molecules 2020, 25(2), 353; https://doi.org/10.3390/molecules25020353
Received: 13 December 2019 / Revised: 11 January 2020 / Accepted: 13 January 2020 / Published: 15 January 2020
As a primary strategy for production of biological drugs, recombinant proteins produced by transient transfection of mammalian cells are essential for both basic research and industrial production. Here, we established a high-throughput screening platform for improving the expression levels of recombinant proteins. In total, 10,011 small molecule compounds were screened through our platform. After two rounds of screening, we identified two compounds, Apicidin and M-344, that significantly enhanced recombinant protein expression. Both of the selected compounds were histone deacetylase inhibitors, suggesting that the two small molecules increased the expression levels of recombinant proteins by promoting histone acetylation. Moreover, both molecules showed low cytotoxicity. Therefore, our findings suggest that these small molecules may have wide applications in the future. View Full-Text
Keywords: small molecules; high-throughput screening; recombinant protein small molecules; high-throughput screening; recombinant protein
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MDPI and ACS Style

Chang, J.; Chen, X.; Wang, R.; Shi, R.; Wang, X.; Lu, W.; Ma, S.; Xia, Q. High-Throughput Screening Identifies Two Novel Small Molecule Enhancers of Recombinant Protein Expression. Molecules 2020, 25, 353.

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