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Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells (Running Title: Casticin Induces DNA Damage in Lung Cancer Cells)

1
Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan
2
Department of Physiology, College of Medicine, China Medical University, Taichung 404, Taiwan
3
Department of Medical Research, China Medical University Hospital, Taichung 404, Taiwan
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Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan
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Department of Respiratory Therapy, China Medical University, Taichung 404, Taiwan
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Department of Internal Medicine, China Medical University Hospital, Taichung 404, Taiwan
*
Authors to whom correspondence should be addressed.
Both authors contributed equally to this work.
Molecules 2020, 25(2), 341; https://doi.org/10.3390/molecules25020341
Received: 20 December 2019 / Accepted: 9 January 2020 / Published: 15 January 2020
Casticin was obtained from natural plants, and it has been shown to exert biological functions; however, no report concerns the induction of DNA damage and repair in human lung cancer cells. The objective of this study was to investigate the effects and molecular mechanism of casticin on DNA damage and repair in human lung cancer A549 cells. Cell viability was determined by flow cytometric assay. The DNA damage was evaluated by 4’,6-diamidino-2-phenylindole (DAPI) staining and electrophoresis which included comet assay and DNA gel electrophoresis. The protein levels associated with DNA damage and repair were analyzed by western blotting. The expression and translocation of p-H2A.X were observed by confocal laser microscopy. Casticin reduced total viable cell number and induced DNA condensation, fragmentation, and damage in A549 cells. Furthermore, casticin increased p-ATM at 6 h and increased p-ATR and BRCA1 at 6–24 h treatment but decreased p-ATM at 24–48 h, as well as decreased p-ATR and BRCA1 at 48 h. Furthermore, casticin decreased p-p53 at 6–24 h but increased at 48 h. Casticin increased p-H2A.X and MDC1 at 6–48 h treatment. In addition, casticin increased PARP (cleavage) at 6, 24, and 48 h treatment, DNA-PKcs and MGMT at 48 h in A549 cells. Casticin induced the expressions and nuclear translocation of p-H2AX in A549 cells by confocal laser microscopy. Casticin reduced cell number through DNA damage and condensation in human lung cancer A549 cells. View Full-Text
Keywords: casticin; human lung cancer A549 cells; DNA damage; DNA condensation and repair casticin; human lung cancer A549 cells; DNA damage; DNA condensation and repair
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Cheng, Z.-Y.; Hsiao, Y.-T.; Huang, Y.-P.; Peng, S.-F.; Huang, W.-W.; Liu, K.-C.; Hsia, T.-C.; Way, T.-D.; Chung, J.-G. Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells (Running Title: Casticin Induces DNA Damage in Lung Cancer Cells). Molecules 2020, 25, 341.

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