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Article

A Bioactive Olive Pomace Extract Prevents the Death of Murine Cortical Neurons Triggered by NMDAR Over-Activation

1
Department of Experimental Medicine (DIMES), University of Genoa, Viale Benedetto XV-1, 16132 Genova, Italy
2
Department of Civil, Chemical and Environmental Engineering, University of Genoa, Pole of Chemical Engineering, via Opera Pia 15, 16145 Genoa, Italy
3
Centre of Excellence for Biomedical Research (CEBR), University of Genoa, Viale Benedetto XV 9, 16132 Genova, Italy
4
IRCCS Ospedale Policlinico San Martino, 16132 Genova, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Cristina Forzato
Molecules 2020, 25(19), 4385; https://doi.org/10.3390/molecules25194385
Received: 18 August 2020 / Revised: 17 September 2020 / Accepted: 21 September 2020 / Published: 24 September 2020
We have recently demonstrated that bioactive molecules, extracted by high pressure and temperature from olive pomace, counteract calcium-induced cell damage to different cell lines. Here, our aim was to study the effect of the same extract on murine cortical neurons, since the preservation of the intracellular Ca2+-homeostasis is essential for neuronal function and survival. Accordingly, we treated neurons with different stimuli in order to evoke cytotoxic glutamatergic activation. In these conditions, the high-pressure and temperature extract from olive pomace (HPTOPE) only abolished the effects of N-methyl-d-aspartate (NMDA). Particularly, we observed that HPTOPE was able to promote the neuron rescue from NMDA-induced cell death. Moreover, we demonstrated that HPTOPE is endowed with the ability to maintain the intracellular Ca2+-homeostasis following NMDA receptor overactivation, protecting neurons from Ca2+-induced adverse effects, including aberrant calpain proteolytic activity. Moreover, we highlight the importance of the extraction conditions used that, without producing toxic molecules, allow us to obtain protecting molecules belonging to proanthocyanidin derivatives like procyanidin B2. In conclusion, we can hypothesize that HPTOPE, due to its functional and nontoxic properties on neuronal primary culture, can be utilized for future therapeutic interventions for neurodegeneration. View Full-Text
Keywords: calcium homeostasis; NMDAR; neuron primary culture; calpain; olive pomace extract; proanthocyanidins calcium homeostasis; NMDAR; neuron primary culture; calpain; olive pomace extract; proanthocyanidins
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MDPI and ACS Style

Franchi, A.; Pedrazzi, M.; Casazza, A.A.; Millo, E.; Damonte, G.; Salis, A.; Liessi, N.; Onofri, F.; Marte, A.; Casagrande, S.; De Tullio, R.; Perego, P.; Averna, M. A Bioactive Olive Pomace Extract Prevents the Death of Murine Cortical Neurons Triggered by NMDAR Over-Activation. Molecules 2020, 25, 4385. https://doi.org/10.3390/molecules25194385

AMA Style

Franchi A, Pedrazzi M, Casazza AA, Millo E, Damonte G, Salis A, Liessi N, Onofri F, Marte A, Casagrande S, De Tullio R, Perego P, Averna M. A Bioactive Olive Pomace Extract Prevents the Death of Murine Cortical Neurons Triggered by NMDAR Over-Activation. Molecules. 2020; 25(19):4385. https://doi.org/10.3390/molecules25194385

Chicago/Turabian Style

Franchi, Alice, Marco Pedrazzi, Alessandro Alberto Casazza, Enrico Millo, Gianluca Damonte, Annalisa Salis, Nara Liessi, Franco Onofri, Antonella Marte, Silvia Casagrande, Roberta De Tullio, Patrizia Perego, and Monica Averna. 2020. "A Bioactive Olive Pomace Extract Prevents the Death of Murine Cortical Neurons Triggered by NMDAR Over-Activation" Molecules 25, no. 19: 4385. https://doi.org/10.3390/molecules25194385

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