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Article

Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B12 Derivatives

1
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland
2
Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland
3
Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Giuliano Siligardi
Molecules 2020, 25(19), 4386; https://doi.org/10.3390/molecules25194386
Received: 31 August 2020 / Revised: 21 September 2020 / Accepted: 23 September 2020 / Published: 24 September 2020
This work demonstrates resonance Raman optical activity (RROA) spectra of three truncated vitamin B12 derivatives modified within the nucleotide loop. Since truncated cobalamins possess sufficiently high rotational strength in the range of ROA excitation (532 nm), it was possible to record their spectra in the resonance condition. They showed several distinct spectral features allowing for the distinguishing of studied compounds, in contrast to other methods, i.e., UV-Vis absorption, electronic circular dichroism, and resonance Raman spectroscopy. The improved capacity of the RROA method is based here on the excitation of molecules via more than two electronic states, giving rise to the bisignate RROA spectrum, significantly distinct from a parent Raman spectrum. This observation is an important step in the dissemination of using RROA spectroscopy in studying the complex structure of corrinoids which may prove crucial for a better understanding of their biological role. View Full-Text
Keywords: vitamin B12; cobinamide; chirality; resonance ROA; resonance Raman spectroscopy; ECD vitamin B12; cobinamide; chirality; resonance ROA; resonance Raman spectroscopy; ECD
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MDPI and ACS Style

Machalska, E.; Zajac, G.; Halat, M.; Wierzba, A.J.; Gryko, D.; Baranska, M. Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B12 Derivatives. Molecules 2020, 25, 4386. https://doi.org/10.3390/molecules25194386

AMA Style

Machalska E, Zajac G, Halat M, Wierzba AJ, Gryko D, Baranska M. Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B12 Derivatives. Molecules. 2020; 25(19):4386. https://doi.org/10.3390/molecules25194386

Chicago/Turabian Style

Machalska, Ewa, Grzegorz Zajac, Monika Halat, Aleksandra J. Wierzba, Dorota Gryko, and Malgorzata Baranska. 2020. "Resonance Raman Optical Activity Spectroscopy in Probing Structural Changes Invisible to Circular Dichroism Spectroscopy: A Study on Truncated Vitamin B12 Derivatives" Molecules 25, no. 19: 4386. https://doi.org/10.3390/molecules25194386

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