Next Article in Journal
Spatial Frequency Response of Epoxy-Based Volume Holographic Recording Material
Next Article in Special Issue
Analysis of Enantiomers in Products of Food Interest
Previous Article in Journal
Comparing Gly11/dAla11-Replacement vs. the in-Situ Neprilysin-Inhibition Approach on the Tumor-targeting Efficacy of the 111In-SB3/111In-SB4 Radiotracer Pair
Previous Article in Special Issue
Fatty Acid, Lipid Classes and Phospholipid Molecular Species Composition of the Marine Clam Meretrix lyrata (Sowerby 1851) from Cua Lo Beach, Nghe An Province, Vietnam
Article Menu

Export Article

Open AccessArticle
Molecules 2019, 24(6), 1017; https://doi.org/10.3390/molecules24061017

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice

1
BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France
2
Department of chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan
3
Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore 54000, Pakistan
4
Université de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Sensors and Biosensors Group, Campus Universitaire de Tunis El Manar, Tunis 2092, Tunisia
*
Authors to whom correspondence should be addressed.
Received: 6 February 2019 / Revised: 8 March 2019 / Accepted: 9 March 2019 / Published: 13 March 2019
(This article belongs to the Special Issue Advances in Food Analysis)
Full-Text   |   PDF [2088 KB, uploaded 13 March 2019]   |  

Abstract

In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH2-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electrochemical impedance spectroscopy, the main analytical technique for the quantitative detection of PAT. Encouragingly, a good linear range between 1 and 25 ng was obtained. The limit of detection and limit of quantification was 2.8 ng L−1 and 4.0 ng L−1, respectively. Selectivity of the aptasensor was confirmed with mycotoxins commonly occurring in food. The developed apta-assay was also applied to a real sample, i.e., fresh apple juice spiked with PAT, and toxin recovery up to 99% was observed. The results obtained validated the suitability and selectivity of the developed apta-assay for the identification and quantification of PAT in real food samples. View Full-Text
Keywords: impedimetric aptasensor; screen-printed interface; bifunctional polymer arms; PAT detection; apple juice impedimetric aptasensor; screen-printed interface; bifunctional polymer arms; PAT detection; apple juice
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Khan, R.; Ben Aissa, S.; Sherazi, T.A.; Catanante, G.; Hayat, A.; Marty, J.L. Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice. Molecules 2019, 24, 1017.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top