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Molecules 2019, 24(6), 1017;

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France
Department of chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan
Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore 54000, Pakistan
Université de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Sensors and Biosensors Group, Campus Universitaire de Tunis El Manar, Tunis 2092, Tunisia
Authors to whom correspondence should be addressed.
Received: 6 February 2019 / Revised: 8 March 2019 / Accepted: 9 March 2019 / Published: 13 March 2019
(This article belongs to the Special Issue Advances in Food Analysis)
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In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH2-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electrochemical impedance spectroscopy, the main analytical technique for the quantitative detection of PAT. Encouragingly, a good linear range between 1 and 25 ng was obtained. The limit of detection and limit of quantification was 2.8 ng L−1 and 4.0 ng L−1, respectively. Selectivity of the aptasensor was confirmed with mycotoxins commonly occurring in food. The developed apta-assay was also applied to a real sample, i.e., fresh apple juice spiked with PAT, and toxin recovery up to 99% was observed. The results obtained validated the suitability and selectivity of the developed apta-assay for the identification and quantification of PAT in real food samples. View Full-Text
Keywords: impedimetric aptasensor; screen-printed interface; bifunctional polymer arms; PAT detection; apple juice impedimetric aptasensor; screen-printed interface; bifunctional polymer arms; PAT detection; apple juice

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Khan, R.; Ben Aissa, S.; Sherazi, T.A.; Catanante, G.; Hayat, A.; Marty, J.L. Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice. Molecules 2019, 24, 1017.

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