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Open AccessArticle

Engineering of Antimicrobial Surfaces by Using Temporin Analogs to Tune the Biocidal/antiadhesive Effect

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Sorbonne Université, CNRS, Laboratoire de Réactivité de Surface, LRS UMR CNRS 7197, F-75252 Paris, France
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Sorbonne Université, CNRS, Institut de Biologie Paris-Seine, IBPS, F-75252 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editor: Naresh Kumar
Molecules 2019, 24(4), 814; https://doi.org/10.3390/molecules24040814
Received: 14 January 2019 / Revised: 19 February 2019 / Accepted: 20 February 2019 / Published: 24 February 2019
(This article belongs to the Special Issue Recent Advances in Antimicrobial Biomaterials)
Proliferation of resistant bacteria on biomaterials is a major problem leading to nosocomial infections. Due to their broad-spectrum activity and their ability to disrupt bacterial membranes through a rapid membranolytic mechanism, antimicrobial peptides (AMPs) are less susceptible to the development of bacterial resistance and therefore represent good candidates for surface coating strategies to prevent biofilm formation. In this study, we report on the covalent immobilization of temporin-SHa, a small hydrophobic and low cationic antimicrobial peptide exhibiting broad-spectrum activity, and (SHa) analogs on modified gold surfaces. Several analogs derived from SHa with either a carboxamidated ([K3]SHa, d-[K3]SHa) or a carboxylated C-terminus ([K3]SHa-COOH) were used to achieve peptide grafting on gold surfaces modified by a thiolated self-assembled monolayer (SAM). Surface functionalization was characterized by polarization modulation infrared reflection absorption spectroscopy (PM-RAIRS) and X-ray photoemission spectroscopy (XPS). The antibacterial properties of the temporin-functionalized surfaces were tested against the Gram-positive Listeria ivanovii. Direct visualization of the peptide effects on the bacterial membrane was investigated by scanning electron microscopy equipped with a field emission gun (SEM-FEG). All active temporin analogs were successfully grafted and display significant antibacterial activity (from 80 to 90% killing efficiency) in addition to a 2-fold decrease of bacterial adhesion when all d-SHa analogs were used. View Full-Text
Keywords: temporin-SHa; SHa analogs; antimicrobial peptides; gold surface functionalization; antibacterial/antiadhesive activity temporin-SHa; SHa analogs; antimicrobial peptides; gold surface functionalization; antibacterial/antiadhesive activity
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MDPI and ACS Style

Oger, P.-C.; Piesse, C.; Ladram, A.; Humblot, V. Engineering of Antimicrobial Surfaces by Using Temporin Analogs to Tune the Biocidal/antiadhesive Effect. Molecules 2019, 24, 814.

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