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Open AccessArticle

Dual Insecticidal Effects of Adenanthera pavonina Kunitz-Type Inhibitor on Plodia interpunctella is Mediated by Digestive Enzymes Inhibition and Chitin-Binding Properties

1
Universidade Federal de Grande Dourados, Dourados, Mato Grosso do Sul, MS, 79825-070, Brazil
2
S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, 79117-900, Brazil
3
Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal da Paraíba, João Pessoa, PB, 58059-900, Brazil
4
Centro de Análises Bioquímica e Proteômicas, Programa de Pós Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, 70790-160, Brazil
5
Programa de Pós-Graduação em Patologia Molecular, Faculdade de Medicina, Universidade de Brasília, Brasília, DF, 70910-900, Brazil
6
Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, 59078-900, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Massimiliano Fenice
Molecules 2019, 24(23), 4344; https://doi.org/10.3390/molecules24234344
Received: 19 September 2019 / Revised: 10 October 2019 / Accepted: 14 October 2019 / Published: 28 November 2019
(This article belongs to the Special Issue Advances in Chitin and Chitosan Science)
The Indianmeal moth, Plodia interpunctella, is one of the most damaging pests of stored products. We investigated the insecticidal properties of ApKTI, a Kunitz trypsin inhibitor from Adenanthera pavonina seeds, against P. interpunctella larvae through bioassays with artificial diet. ApKTI-fed larvae showed reduction of up to 88% on larval weight and 75% in survival. Trypsin enzymes extracted from P. interpunctella larvae were inhibited by ApKTI, which also demonstrated capacity to bind to chitin. Kinetic studies revealed a non-competitive inhibition mechanism of ApKTI for trypsin, which were further corroborated by molecular docking studies. Furthermore, we have demonstrated that ApKTI exhibits a hydrophobic pocket near the reactive site loop probably involved in chitin interactions. Taken together, these data suggested that the insecticidal activity of ApKTI for P. interpunctella larvae involves a dual and promiscuous mechanisms biding to two completely different targets. Both processes might impair the P. interpunctella larval digestive process, leading to larvae death before reaching the pupal stage. Further studies are encouraged using ApKTI as a biotechnological tool to control insect pests in field conditions. View Full-Text
Keywords: non-competitive inhibitor; trypsin inhibitor; peritrophic membrane non-competitive inhibitor; trypsin inhibitor; peritrophic membrane
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MDPI and ACS Style

de Oliveira, C.F.R.; de Oliveira Flores, T.M.; Henrique Cardoso, M.; Garcia Nogueira Oshiro, K.; Russi, R.; de França, A.F.J.; dos Santos, E.A.; Luiz Franco, O.; de Oliveira, A.S.; Migliolo, L. Dual Insecticidal Effects of Adenanthera pavonina Kunitz-Type Inhibitor on Plodia interpunctella is Mediated by Digestive Enzymes Inhibition and Chitin-Binding Properties. Molecules 2019, 24, 4344. https://doi.org/10.3390/molecules24234344

AMA Style

de Oliveira CFR, de Oliveira Flores TM, Henrique Cardoso M, Garcia Nogueira Oshiro K, Russi R, de França AFJ, dos Santos EA, Luiz Franco O, de Oliveira AS, Migliolo L. Dual Insecticidal Effects of Adenanthera pavonina Kunitz-Type Inhibitor on Plodia interpunctella is Mediated by Digestive Enzymes Inhibition and Chitin-Binding Properties. Molecules. 2019; 24(23):4344. https://doi.org/10.3390/molecules24234344

Chicago/Turabian Style

de Oliveira, Caio F.R.; de Oliveira Flores, Taylla M.; Henrique Cardoso, Marlon; Garcia Nogueira Oshiro, Karen; Russi, Raphael; de França, Anderson F.J.; dos Santos, Elizeu A.; Luiz Franco, Octávio; de Oliveira, Adeliana S.; Migliolo, Ludovico. 2019. "Dual Insecticidal Effects of Adenanthera pavonina Kunitz-Type Inhibitor on Plodia interpunctella is Mediated by Digestive Enzymes Inhibition and Chitin-Binding Properties" Molecules 24, no. 23: 4344. https://doi.org/10.3390/molecules24234344

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