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Molecules 2019, 24(2), 244; https://doi.org/10.3390/molecules24020244

Production of a Novel Tetrahydroxynaphthalene (THN) Derivative from Nocardia sp. CS682 by Metabolic Engineering and Its Bioactivities

1
Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sun Moon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Korea
2
Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, 70 Sun Moon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, Korea
These authors contributed equally.
*
Author to whom correspondence should be addressed.
Received: 13 November 2018 / Revised: 26 December 2018 / Accepted: 8 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Drug Discovery from Microorganisms)
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Abstract

Nargenicin A1 is major secondary metabolite produced by Nocardia sp. CS682, with an effective antibacterial activity against various Gram-positive bacteria. Most Nocardia spp. have metabolic ability to produce compounds of diverse nature, so one-strain-many-compounds (OSMAC) approach can be applied for obtaining versatile compounds from these strains. In this study, we characterized a novel 1, 3, 6, 8-tetrahydroxynaphthalene (THN) derivative by metabolic engineering approach leading to the inactivation of nargenicin A1 biosynthesis. By using genome mining, metabolite profiling, and bioinformatics, the biosynthetic gene cluster and biosynthetic mechanism were elucidated. Further, the antibacterial, anticancer, melanin formation, and UV protective properties for isolated THN compound were performed. The compound did not exhibit significant antibacterial and cytotoxic activities, but it exhibited promising UV protection effects. Thus, metabolic engineering is an effective strategy for discovering novel bioactive molecules. View Full-Text
Keywords: Nocardia sp. CS682; Type III Polyketide Synthase; tetrahydroxynaphthalene biosynthesis; UV-protection Nocardia sp. CS682; Type III Polyketide Synthase; tetrahydroxynaphthalene biosynthesis; UV-protection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Mishra, R.; Dhakal, D.; Han, J.M.; Lim, H.N.; Jung, H.J.; Yamaguchi, T.; Sohng, J.K. Production of a Novel Tetrahydroxynaphthalene (THN) Derivative from Nocardia sp. CS682 by Metabolic Engineering and Its Bioactivities. Molecules 2019, 24, 244.

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