Next Article in Journal
Hybrid Geopolymers from Fly Ash and Polysiloxanes
Next Article in Special Issue
Chlojaponilactone B Attenuates Lipopolysaccharide-Induced Inflammatory Responses by Suppressing TLR4-Mediated ROS Generation and NF-κB Signaling Pathway
Previous Article in Journal
Structural, Impedance, and EDLC Characteristics of Proton Conducting Chitosan-Based Polymer Blend Electrolytes with High Electrochemical Stability
Previous Article in Special Issue
Therapeutic Use of Scoparia dulcis Reduces the Progression of Experimental Osteoarthritis
Article

Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation

1
Chemistry and Biochemistry Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, Canada
2
Ecosystem Science and Management Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, Canada
3
Food and Nutritional Sciences Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
4
Northern Medical Program, University of Northern British Columbia, Prince George, BC V2N 4Z9, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Maria da Graça Costa G. Miguel
Molecules 2019, 24(19), 3509; https://doi.org/10.3390/molecules24193509
Received: 9 September 2019 / Accepted: 24 September 2019 / Published: 27 September 2019
(This article belongs to the Special Issue Anti-Inflammatory Activity of Natural Products)
The aim of this study was to investigate the anti-inflammatory activity of a previously un-studied wild mushroom, Echinodontium tinctorium, collected from the forests of north-central British Columbia. The lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used to study the in vitro anti-inflammatory activity. The crude alkaline extract demonstrated potent anti-inflammatory activity, and was further purified using a “bio-activity-guided-purification” approach. The size-exclusion and ion-exchange chromatography yielded a water-soluble anti-inflammatory polysaccharide (AIPetinc). AIPetinc has an average molecular weight of 5 kDa, and is a heteroglucan composed of mainly glucose (88.6%) with a small amount of galactose (4.0%), mannose (4.4%), fucose (0.7%), and xylose (2.3%). In in vivo settings, AIPetinc restored the histamine-induced inflammatory event in mouse gluteus maximus muscle, thus confirming its anti-inflammatory activity in an animal model. This study constitutes the first report on the bioactivity of Echinodontium tinctorium, and highlights the potential medicinal benefits of fungi from the wild forests of northern British Columbia. Furthermore, it also reiterates the need to explore natural resources for alternative treatment to modern world diseases. View Full-Text
Keywords: Echinodontium tinctorium; polysaccharide; anti-inflammation; mushroom; British Columbia Echinodontium tinctorium; polysaccharide; anti-inflammation; mushroom; British Columbia
Show Figures

Figure 1

MDPI and ACS Style

Javed, S.; Li, W.M.; Zeb, M.; Yaqoob, A.; Tackaberry, L.E.; Massicotte, H.B.; Egger, K.N.; Cheung, P.C.K.; Payne, G.W.; Lee, C.H. Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation. Molecules 2019, 24, 3509. https://doi.org/10.3390/molecules24193509

AMA Style

Javed S, Li WM, Zeb M, Yaqoob A, Tackaberry LE, Massicotte HB, Egger KN, Cheung PCK, Payne GW, Lee CH. Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation. Molecules. 2019; 24(19):3509. https://doi.org/10.3390/molecules24193509

Chicago/Turabian Style

Javed, Sumreen, Wai M. Li, Mehreen Zeb, Almas Yaqoob, Linda E. Tackaberry, Hugues B. Massicotte, Keith N. Egger, Peter C.K. Cheung, Geoffrey W. Payne, and Chow H. Lee 2019. "Anti-Inflammatory Activity of the Wild Mushroom, Echinodontium tinctorium, in RAW264.7 Macrophage Cells and Mouse Microcirculation" Molecules 24, no. 19: 3509. https://doi.org/10.3390/molecules24193509

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop