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Open AccessArticle

Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume

National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
Authors to whom correspondence should be addressed.
Molecules 2019, 24(17), 3159;
Received: 18 July 2019 / Revised: 21 August 2019 / Accepted: 28 August 2019 / Published: 30 August 2019
(This article belongs to the Special Issue Recent Advances in Food and Natural Product Analysis)
To explore the transformation mechanisms of free gastrodin and combined gastrodin before and after steaming of Gastrodia elata (G. elata), a fresh G. elata sample was processed by the traditional steaming method prescribed by Chinese Pharmacopoeia (2015 version), and HPLC-ESI-TOF/MS method was used to identify the chemical composition in steamed and fresh G. elata. Finally, 25 components were identified in G. elata based on the characteristic fragments of the compounds and the changes of the 25 components of fresh and steamed G. elata were compared by the relative content. Hydrolysis experiments and enzymatic hydrolysis experiments of 10 monomer compounds simulating the G. elata steaming process were carried out for the first time. As a result, hydrolysis experiments proved that free gastrodin or p-hydroxybenzyl alcohol could be obtained by breaking ester bond or ether bond during the steaming process of G. elata. Enzymatic experiments showed that steaming played an important role in the protection of gastrodin, confirming the hypothesis that steaming can promote the conversion of chemical constituents of G. elata—inhibiting enzymatic degradation. This experiment clarified the scientific mechanism of the traditional steaming method of G. elata and provided reference for how to apply G. elata decoction to some extent. View Full-Text
Keywords: Gastrodia elata; steaming; gastrodin; parishin A; hydrolysis Gastrodia elata; steaming; gastrodin; parishin A; hydrolysis
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MDPI and ACS Style

Li, Y.; Liu, X.-Q.; Liu, S.-S.; Liu, D.-H.; Wang, X.; Wang, Z.-M. Transformation Mechanisms of Chemical Ingredients in Steaming Process of Gastrodia elata Blume. Molecules 2019, 24, 3159.

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