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Feasibility of Utilizing Stable-Isotope Dimethyl Labeling in Liquid Chromatography–Tandem Mass Spectrometry-Based Determination for Food Allergens—Case of Kiwifruit

Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei 10673, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally in this work.
Academic Editor: Chi-Fai Chau
Molecules 2019, 24(10), 1920; https://doi.org/10.3390/molecules24101920 (registering DOI)
Received: 1 May 2019 / Revised: 17 May 2019 / Accepted: 17 May 2019 / Published: 18 May 2019
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Abstract

Stable-isotope dimethyl labeling is a highly reactive and cost-effective derivatization procedure that could be utilized in proteomics analysis. In this study, a liquid chromatography– tandem mass spectrometry in multiple reaction monitoring mode (LC-MS-MRM) platform for the quantification of kiwi allergens was first developed using this strategy. Three signature peptides for target allergens Act d 1, Act d 5, and Act d 11 were determined and were derivatized with normal and deuterated formaldehyde as external calibrants and internal standards, respectively. The results showed that sample preparation with the phenol method provided comprehensive protein populations. Recoveries at four different levels ranging from 72.5–109.3% were achieved for the H-labeled signature peptides of Act d 1 (SPA1-H) and Act d 5 (SPA5-H) with precision ranging from 1.86–9.92%. The limit of quantification (LOQ) was set at 8 pg mL−1 for SPA1-H and at 8 ng mL−1 for SPA5-H. The developed procedure was utilized to analyze seven kinds of hand-made kiwi foods containing 0.0175–0.0515 mg g−1 of Act d 1 and 0.0252–0.0556 mg g−1 of Act d 5. This study extended the applicability of stable-isotope dimethyl labeling to the economical and precise determination of food allergens and peptides. View Full-Text
Keywords: stable-isotope dimethyl labeling; liquid chromatography–tandem mass spectrometry; food allergen; kiwifruit stable-isotope dimethyl labeling; liquid chromatography–tandem mass spectrometry; food allergen; kiwifruit
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Shih, Y.-C.; Hsiao, J.-T.; Sheu, F. Feasibility of Utilizing Stable-Isotope Dimethyl Labeling in Liquid Chromatography–Tandem Mass Spectrometry-Based Determination for Food Allergens—Case of Kiwifruit. Molecules 2019, 24, 1920.

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