Aza-Amino Acids Disrupt β-Sheet Secondary Structures
AbstractCα to N substitution in aza-amino acids imposes local conformational constraints, changes in hydrogen bonding properties, and leads to adaptive chirality at the nitrogen atom. These properties can be exploited in mimicry and stabilization of peptide secondary structures and self-assembly. Here, the effect of a single aza-amino acid incorporation located in the upper β-strand at a hydrogen-bonded (HB) site of a β-hairpin model peptide (H-Arg-Tyr-Val-Glu-Val-d-Pro-Gly-Orn-Lys-Ile-Leu-Gln-NH2) is reported. Specifically, analogs in which valine3 was substituted for aza-valine3 or aza-glycine3 were synthesized, and their β-hairpin stabilities were examined using Nuclear Magnetic Resonance (NMR) spectroscopy. The azapeptide analogs were found to destabilize β-hairpin formation compared to the parent peptide. The aza-valine3 residue was more disruptive of β-hairpin geometry than its aza-glycine3 counterpart. View Full-Text
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McMechen, M.A.; Willis, E.L.; Gourville, P.C.; Proulx, C. Aza-Amino Acids Disrupt β-Sheet Secondary Structures. Molecules 2019, 24, 1919.
McMechen MA, Willis EL, Gourville PC, Proulx C. Aza-Amino Acids Disrupt β-Sheet Secondary Structures. Molecules. 2019; 24(10):1919.Chicago/Turabian Style
McMechen, Michael A.; Willis, Evan L.; Gourville, Preston C.; Proulx, Caroline. 2019. "Aza-Amino Acids Disrupt β-Sheet Secondary Structures." Molecules 24, no. 10: 1919.
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