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Article

Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes

Department of Food and Nutrition, Gachon University, Gyeonggi-do 13120, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Molecules 2019, 24(10), 1848; https://doi.org/10.3390/molecules24101848
Received: 12 April 2019 / Revised: 4 May 2019 / Accepted: 8 May 2019 / Published: 14 May 2019
(This article belongs to the Special Issue Anthocyanins: Recent Progress in Health Benefits Studies)
Delphinidin-3-O-β-glucoside (D3G) is a health-promoting anthocyanin whose anti-obesity activity has not yet been thoroughly investigated. We examined the effects of D3G on adipogenesis and lipogenesis in 3T3-L1 adipocytes and primary white adipocytes using real-time RT-PCR and immunoblot analysis. D3G significantly inhibited the accumulation of lipids in a dose-dependent manner without displaying cytotoxicity. In the 3T3-L1 adipocytes, D3G downregulated the expression of key adipogenic and lipogenic markers, which are known as peroxisome proliferator-activated receptor gamma (PPARγ), sterol regulatory element-binding transcription factor 1 (SREBP1), CCAAT/enhancer-binding protein alpha (C/EBPα), and fatty acid synthase (FAS). Moreover, the relative protein expression of silent mating type information regulation 2 homolog 1 (SIRT1) and carnitine palmitoyltransferase-1 (CPT-1) were increased, alongside reduced lipid levels and the presence of several small lipid droplets. Furthermore, D3G increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), which suggests that D3G may play a role in AMPK and ACC activation in adipocytes. Our data indicate that D3G attenuates adipogenesis and promotes lipid metabolism by activating AMPK-mediated signaling, and, hence, could have a therapeutic role in the management and treatment of obesity. View Full-Text
Keywords: anthocyanin; delphinidin-3-O-β-glucoside; 3T3-L1 adipocytes; primary white adipocytes; adipogenesis; differentiation anthocyanin; delphinidin-3-O-β-glucoside; 3T3-L1 adipocytes; primary white adipocytes; adipogenesis; differentiation
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MDPI and ACS Style

Park, M.; Sharma, A.; Lee, H.-J. Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes. Molecules 2019, 24, 1848. https://doi.org/10.3390/molecules24101848

AMA Style

Park M, Sharma A, Lee H-J. Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes. Molecules. 2019; 24(10):1848. https://doi.org/10.3390/molecules24101848

Chicago/Turabian Style

Park, Miey, Anshul Sharma, and Hae-Jeung Lee. 2019. "Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes" Molecules 24, no. 10: 1848. https://doi.org/10.3390/molecules24101848

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