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New Bacterial Phytase through Metagenomic Prospection

Graduate Program in Agricultural Sciences, Universidade Estadual da Paraíba (UEPB), Campina Grande/PB 58429-500, Brazil
Department of Biology, Universidade Estadual da Paraíba (UEPB)
Department of Biology and Graduate Program in Agricultural Sciences, Universidade Estadual da Paraíba (UEPB), Campina Grande/PB 58429-500, Brazil
Author to whom correspondence should be addressed.
Molecules 2018, 23(2), 448;
Received: 23 January 2018 / Revised: 7 February 2018 / Accepted: 15 February 2018 / Published: 17 February 2018
(This article belongs to the Section Molecular Diversity)
PDF [3157 KB, uploaded 26 February 2018]


Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology. View Full-Text
Keywords: crop residues; functional metagenomics; phytasic activity crop residues; functional metagenomics; phytasic activity

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Farias, N.; Almeida, I.; Meneses, C. New Bacterial Phytase through Metagenomic Prospection. Molecules 2018, 23, 448.

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