Molecules 2017, 22(6), 982; https://doi.org/10.3390/molecules22060982
The Complete Chloroplast Genome Sequences of Fritillaria ussuriensis Maxim. and Fritillaria cirrhosa D. Don, and Comparative Analysis with Other Fritillaria Species
Inkyu Park 1, Wook Jin Kim 1, Sang-Min Yeo 1, Goya Choi 1, Young-Min Kang 1, Renzhe Piao 2 and Byeong Cheol Moon 1,*
1
K-Herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea
2
Department of Agronomy, Yanbian University Agriculture College, Yanji 133002, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 6 March 2017 / Revised: 2 June 2017 / Accepted: 10 June 2017 / Published: 13 June 2017
(This article belongs to the Section Molecular Diversity)
Abstract
The genus Fritillaria belongs to the widely distributed Liliaceae. The bulbs of Fritillaria, F. ussuriensis and F. cirrhosa are valuable herbaceous medicinal ingredients. However, they are still used indiscriminately in herbal medicine. Identification and molecular phylogenic analysis of Fritillaria species are therefore required. Here, we report the complete chloroplast (CP) genome sequences of F. ussuriensis and F. cirrhosa. The two Fritillaria CP genomes were 151,524 and 151,083 bp in length, respectively, and each included a pair of inverted repeated regions (52,678 and 52,156 bp) that was separated by a large single copy region (81,732 and 81,390 bp), and a small single copy region (17,114 and 17,537 bp). A total of 111 genes in F. ussuriensis and 112 in F. cirrhosa comprised 77 protein-coding regions in F. ussuriensis and 78 in F. cirrhosa, 30 transfer RNA (tRNA) genes, and four ribosomal RNA (rRNA) genes. The gene order, content, and orientation of the two Fritillaria CP genomes exhibited the general structure of flowering plants, and were similar to those of other Fritillaria species. Comparison of the six Fritillaria species’ CP genomes indicated seven highly divergent regions in intergenic spacers and in the matK, rpoC1, rpoC2, ycf1, ycf2, ndhD, and ndhF coding regions. We established the position of the six species through phylogenic analysis. The complete chloroplast genome sequences of the two Fritillaria species and a comparison study are useful genomic information for identifying and for studying the phylogenetic relationship among Fritillaria species within the Liliaceae. View Full-TextKeywords:
Fritillaria ussuriensis; Fritillaria cirrhosa; chloroplast genome; comparative analysis; highly divergent region
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Park, I.; Kim, W.J.; Yeo, S.-M.; Choi, G.; Kang, Y.-M.; Piao, R.; Moon, B.C. The Complete Chloroplast Genome Sequences of Fritillaria ussuriensis Maxim. and Fritillaria cirrhosa D. Don, and Comparative Analysis with Other Fritillaria Species. Molecules 2017, 22, 982.
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