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Open AccessArticle

Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose

1
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China
2
Beijing Chinese Medicine Research Institute, Beijing University of Chinese Medicine, North Third Ring Road No. 11 School Range, Chaoyang District, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Quan-Bin Han
Molecules 2016, 21(11), 1496; https://doi.org/10.3390/molecules21111496
Received: 26 September 2016 / Revised: 2 November 2016 / Accepted: 2 November 2016 / Published: 9 November 2016
(This article belongs to the Special Issue Natural Polysaccharides)
Fuzi has been used to treat diabetic complications for many years in china. In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury. Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated. Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn). The results witnessed the efficiency of this method and seven monosaccharides were tentatively identified, among which fucose was first reported. Simultaneously, m/z 215 can be considered as diagnostic ions to confirm the number of monosaccharides. Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group. After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes. Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot. Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level. Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly. Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage. View Full-Text
Keywords: Fuzi polysaccharides; HPLC-ESI-MSn; monosaccharide composition; Schwann cells; antioxidant; AMPK-PGC-1α pathway Fuzi polysaccharides; HPLC-ESI-MSn; monosaccharide composition; Schwann cells; antioxidant; AMPK-PGC-1α pathway
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MDPI and ACS Style

Wang, B.-B.; Wang, J.-L.; Yuan, J.; Quan, Q.-H.; Ji, R.-F.; Tan, P.; Han, J.; Liu, Y.-G. Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose. Molecules 2016, 21, 1496.

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