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Volume 20
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10.3390/molecules20010293
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Article

Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway

by
Kai Gao
1,†,
Meiyou Liu
1,†,
Jinyi Cao
1,†,
Minna Yao
1,
Yunyang Lu
2,
Jiankang Li
1,
Xiaohe Zhu
1,
Zhifu Yang
1,* and
Aidong Wen
1,*
1
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China
2
Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2015, 20(1), 293-308; https://doi.org/10.3390/molecules20010293
Submission received: 12 November 2014 / Accepted: 19 December 2014 / Published: 24 December 2014
(This article belongs to the Section Medicinal Chemistry)
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Abstract

Oxidative stress plays an important role in Parkinson’s disease and other neurodegenerative disorders. Lycium barbarum polysaccharides (LBP), the main active ingredients extracted from the fruits of Lycium barbarum L., have been shown to be a potent antioxidant. In the present study, we investigated the protective effects, and the possible mechanism of action of LBP against 6-hydroxydopamine (6-OHDA)-induced apoptosis in PC12 cells. Our data demonstrated that LBP significantly reversed the 6-OHDA-induced decrease in cell viability, prevented 6-OHDA-induced changes in condensed nuclei and decreased the percentage of apoptotic cells in a dose-dependent manner. Furthermore, LBP also slowed the accumulation of reactive oxygen species (ROS) and nitric oxide (NO), decreased the level of protein-bound 3-nitrotyrosine (3-NT) and intracellular free Ca2+, and inhibiting the overexpression of nuclear factor κB (NF-κB), neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS). These results demonstrate that LBP prevents 6-OHDA-induced apoptosis in PC12 cells, at least in part through the ROS-NO pathway.
Keywords: Parkinson’s disease; LBP; PC cells; apoptosis; reactive oxygen species; nitric oxide; mitochondrion Parkinson’s disease; LBP; PC cells; apoptosis; reactive oxygen species; nitric oxide; mitochondrion

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MDPI and ACS Style

Gao, K.; Liu, M.; Cao, J.; Yao, M.; Lu, Y.; Li, J.; Zhu, X.; Yang, Z.; Wen, A. Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway. Molecules 2015, 20, 293-308. https://doi.org/10.3390/molecules20010293

AMA Style

Gao K, Liu M, Cao J, Yao M, Lu Y, Li J, Zhu X, Yang Z, Wen A. Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway. Molecules. 2015; 20(1):293-308. https://doi.org/10.3390/molecules20010293

Chicago/Turabian Style

Gao, Kai, Meiyou Liu, Jinyi Cao, Minna Yao, Yunyang Lu, Jiankang Li, Xiaohe Zhu, Zhifu Yang, and Aidong Wen. 2015. "Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway" Molecules 20, no. 1: 293-308. https://doi.org/10.3390/molecules20010293

APA Style

Gao, K., Liu, M., Cao, J., Yao, M., Lu, Y., Li, J., Zhu, X., Yang, Z., & Wen, A. (2015). Protective Effects of Lycium barbarum Polysaccharide on 6-OHDA-Induced Apoptosis in PC12 Cells through the ROS-NO Pathway. Molecules, 20(1), 293-308. https://doi.org/10.3390/molecules20010293

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