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In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria

Nucleus of Research in Sciences and Technology, University of Franca, Armando Salles de Oliveira Road, 201, Franca 1404-600, Brazil
Faculty of Pharmaceutical Sciences of Ribeirão Preto, Laboratory of Pharmacognosy, University of São Paulo, Café Avenue, Ribeirão Preto 14040-903, Brazil
Authors to whom correspondence should be addressed.
Molecules 2013, 18(7), 7865-7872;
Received: 27 May 2013 / Revised: 20 June 2013 / Accepted: 21 June 2013 / Published: 4 July 2013
(This article belongs to the Section Natural Products Chemistry)
We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 µg mL−1) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis. View Full-Text
Keywords: diterpenes; bovine mastitis; antibacterial; ent-copalic acid diterpenes; bovine mastitis; antibacterial; ent-copalic acid
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Fonseca, A.P.; Estrela, F.T.; Moraes, T.S.; Carneiro, L.J.; Bastos, J.K.; Santos, R.A.; Ambrósio, S.R.; Martins, C.H.G.; Veneziani, R.C.S. In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria. Molecules 2013, 18, 7865-7872.

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