In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria
by
Ariana P. Fonseca 1, Fernanda T. Estrela 1, Thaís S. Moraes 1, Luiza J. Carneiro 1, Jairo K. Bastos 2, Raquel A. dos Santos 1, Sérgio R. Ambrósio 1, 
Carlos H. G. Martins 1,* and Rodrigo C. S. Veneziani 1,*
Carlos H. G. Martins 1,* and Rodrigo C. S. Veneziani 1,*
1
Nucleus of Research in Sciences and Technology, University of Franca, Armando Salles de Oliveira Road, 201, Franca 1404-600, Brazil
2
Faculty of Pharmaceutical Sciences of Ribeirão Preto, Laboratory of Pharmacognosy, University of São Paulo, Café Avenue, Ribeirão Preto 14040-903, Brazil
*
Authors to whom correspondence should be addressed.
Molecules 2013, 18(7), 7865-7872; https://doi.org/10.3390/molecules18077865
Received: 27 May 2013 / Revised: 20 June 2013 / Accepted: 21 June 2013 / Published: 4 July 2013
(This article belongs to the Section Natural Products Chemistry)
We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 µg mL−1) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis.
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Keywords:
diterpenes; bovine mastitis; antibacterial; ent-copalic acid
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This is an open access article distributed under the Creative Commons Attribution License
MDPI and ACS Style
Fonseca, A.P.; Estrela, F.T.; Moraes, T.S.; Carneiro, L.J.; Bastos, J.K.; Santos, R.A.; Ambrósio, S.R.; Martins, C.H.G.; Veneziani, R.C.S. In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria. Molecules 2013, 18, 7865-7872.
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