Human Current and Future Model Systems

Topic Information

Dear Colleagues,

Human models are anticipated to become more and more important not only in the field of basic but also, explicitly, translational research. Most animal models cannot mirror human development, physiology, or pathophysiology in a variety of aspects. Nevertheless, the complexity of an organism is in turn badly mimicked in conventional in vitro models. Based on the rising interest in human co-cultures and three-dimensional model systems, it becomes clear that current and future advanced in vitro human platforms will be considered crucial in the field of human research, including pharmacological and toxicological screenings.

Prof. Dr. Stefan Liebau
Dr. Kevin Achberger
Dr. Markus Breunig
Topic Editors

Keywords

Participating Journals

Journal Name	Impact Factor	CiteScore	Launched Year	First Decision (median)	APC
Biomolecules biomolecules	4.8	9.4	2011	18.4 Days	CHF 2700
Cells cells	5.1	9.9	2012	17 Days	CHF 2700
Journal of Developmental Biology jdb	2.2	4.1	2013	20.3 Days	CHF 1800
Journal of Molecular Pathology jmp	-	-	2020	26.5 Days	CHF 1000
Organoids organoids	-	-	2022	19.3 Days	CHF 1000

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All Biomolecules Cells JDB JMP Organoids

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Open AccessArticle

Pancreatic Cancer 3D Cell Line Organoids (CLOs) Maintain the Phenotypic Characteristics of Organoids and Accurately Reflect the Cellular Architecture and Heterogeneity In Vivo

by Sara Noorani, Shannon R. Nelson, Neil T. Conlon, Justine Meiller, Ekaterina Shcheglova, Alice Usai, Jojanneke Stoof, Letizia Palanga, Fiona O’Neill, Sandra Roche, Maura B. Cotter, Niall Swan and Naomi Walsh

Organoids 2022, 1(2), 168-183; https://doi.org/10.3390/organoids1020013 - 12 Dec 2022

Cited by 2 | Viewed by 5557

Abstract

Pancreatic cancer is a highly lethal disease. Therapeutic resistance to chemotherapy is a major cause of treatment failure and recurrence in pancreatic cancer. Organoids derived from cancer stem cells (CSC) are promising models for the advancement of personalised therapeutic responses to inform clinical [...] Read more.

Pancreatic cancer is a highly lethal disease. Therapeutic resistance to chemotherapy is a major cause of treatment failure and recurrence in pancreatic cancer. Organoids derived from cancer stem cells (CSC) are promising models for the advancement of personalised therapeutic responses to inform clinical decisions. However, scaling-up of 3D organoids for high-throughput screening is time-consuming and costly. Here, we successfully developed organoid-derived cell lines (2.5D) from 3D organoids; the cells were then expanded and recapitulated back into organoids known as cell line organoids (CLOs). The 2.5D lines were cultured long term into 2D established cell lines for downstream comparison analysis. Experimental characterisation of the models revealed that the proliferation of CLOs was slightly faster than that of parental organoids. The therapeutic response to chemotherapeutic agents in 3D CLOs and organoids showed a similar responsive profile. Compared to 3D CLOs and organoids, 2D cell lines tended to be less responsive to all the drugs tested. Stem cell marker expression was higher in either 3D CLOs or organoids compared to 2D cell lines. An in vivo tumorigenicity study found CLOs form tumours at a similar rate to organoids and retain enhanced CSC marker expression, indicating the plasticity of CSCs within the in vivo microenvironment. Full article

(This article belongs to the Topic Human Current and Future Model Systems)

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